Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer

Kanemoto, Yuko; Sanada, Fumihiro; Shibata, Kana; Tsunetoshi, Yasuo; Katsuragi, Naruto; Koibuchi, Nobutaka; Yoshinami, Tetsuhiro; Yamamoto, Koichi; Morishita, Ryuichi; Taniyama, Yoshiaki; Shimazu, Kenzo

doi:https://doi.org/10.3390/biom14091093

Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer

Kanemoto, Yuko; Sanada, Fumihiro; Shibata, Kana et al.

Biomolecules, 2024, 14(9), 1093

Number of Access:1,373（2025-08-14 03:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98374

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Title	Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer
Creator	NRID 1000030924930 1000030924930 CreatorName Kanemoto, Yuko
	NRID 1000030722227 1000030722227 researchmap permalink yamato0520 yamato0520 Scopus Author ID 16320033200 16320033200 ORCID 0000-0002-3728-5607 0000-0002-3728-5607 CreatorName Sanada, Fumihiro
	CreatorName Shibata, Kana
	CreatorName Tsunetoshi, Yasuo
	NRID 1000030544506 1000030544506 researchmap permalink katsuragin27 katsuragin27 CreatorName Katsuragi, Naruto
	NRID 1000030456131 1000030456131 researchmap permalink read0154050 read0154050 CreatorName Koibuchi, Nobutaka
	NRID 1000030894240 1000030894240 Scopus Author ID 36009656500 36009656500 researchmap permalink osaka-yoshi osaka-yoshi CreatorName Yoshinami, Tetsuhiro
	NRID 1000000528424 1000000528424 ORCID 0000-0003-0107-9315 0000-0003-0107-9315 researchmap permalink kymt0410 kymt0410 Scopus Author ID 55624482466 55624482466 CreatorName Yamamoto, Koichi
	NRID 1000040291439 1000040291439 researchmap permalink read0051652 read0051652 Scopus Author ID 7102241494 7102241494 CreatorName Morishita, Ryuichi
	NRID 1000060372611 1000060372611 ORCID 0000-0002-6057-7982 0000-0002-6057-7982 researchmap permalink taniyama taniyama CreatorName Taniyama, Yoshiaki
	NRID 1000030448039 1000030448039 researchmap permalink _-23f _-23f Scopus Author ID 7005224756 7005224756 CreatorName Shimazu, Kenzo
Subject	alternative splicing variants
	breast cancer
	extracellular matrix protein
	periostin
Description	Kanemoto Y., Sanada F., Shibata K., et al. Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer. Biomolecules 14, 1093 (2024); https://doi.org/10.3390/biom14091093.
Abstract	(1) Background: Periostin (Pn) is a secreted protein found in the extracellular matrix, and it plays a variety of roles in the human body. Physiologically, Pn has a variety of functions, including bone formation and wound healing. However, it has been implicated in the pathogenesis of various malignant tumors and chronic inflammatory diseases. Pn has alternative splicing variants (ASVs), and our previous research revealed that aberrant ASVs contribute to the pathogenesis of breast cancer and heart failure. However, the difference in expression pattern between physiologically expressed Pn-ASVs and those expressed during pathogenesis is not clear. (2) Methods and results: We examined normal and breast cancer tissues, focusing on the Pn-ASVs expression pattern to assess the significance of pathologically expressed Pn-ASVs as potential diagnostic and therapeutic targets. We found that most physiologically expressed Pn isoforms lacked exon 17 and 21. Next, we used human breast cancer and normal adjacent tissue (NAT) to investigate the expression pattern of Pn-ASVs under pathological conditions. Pn-ASVs with exon 21 were significantly increased in tumor tissues compared with NAT. In situ hybridization identified the synthesis of Pn-ASVs with exon 21 in peri-tumoral stromal cells. Additionally, the in vivo bio-distribution of 89Zr-labeled Pn antibody against exon 21 (Pn-21Ab) in mice bearing breast cancer demonstrated selective and specific accumulation in tumors, while Pn-21Ab significantly suppressed tumor growth in the mouse breast cancer model. (3) Conclusions: Together, these data indicate that Pn-ASVs might have potential for use as diagnostic and therapeutic targets for breast cancer.
Publisher	Multidisciplinary Digital Publishing Institute (MDPI)
Source Title	Biomolecules
Volume (Issue)	14(9)
Page	1093
Date of Issued	2024-09-01
Language	English
Handle URL	https://hdl.handle.net/11094/98374
eISSN	2218273X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.3390/biom14091093
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biomolecules. 2024, 14(9), p. 1093
DC.title	Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer
DC.creator	Kanemoto, Yuko
	Sanada, Fumihiro
	Shibata, Kana
	Tsunetoshi, Yasuo
	Katsuragi, Naruto
	Koibuchi, Nobutaka
	Yoshinami, Tetsuhiro
	Yamamoto, Koichi
	Morishita, Ryuichi
	Taniyama, Yoshiaki
	Shimazu, Kenzo
DC.publisher	Multidisciplinary Digital Publishing Institute (MDPI)
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-01
DC.identifier	https://doi.org/10.3390/biom14091093
DC.subject	alternative splicing variants
	breast cancer
	extracellular matrix protein
	periostin
DC.description	Kanemoto Y., Sanada F., Shibata K., et al. Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer. Biomolecules 14, 1093 (2024); https://doi.org/10.3390/biom14091093.
DCTERMS.abstract	(1) Background: Periostin (Pn) is a secreted protein found in the extracellular matrix, and it plays a variety of roles in the human body. Physiologically, Pn has a variety of functions, including bone formation and wound healing. However, it has been implicated in the pathogenesis of various malignant tumors and chronic inflammatory diseases. Pn has alternative splicing variants (ASVs), and our previous research revealed that aberrant ASVs contribute to the pathogenesis of breast cancer and heart failure. However, the difference in expression pattern between physiologically expressed Pn-ASVs and those expressed during pathogenesis is not clear. (2) Methods and results: We examined normal and breast cancer tissues, focusing on the Pn-ASVs expression pattern to assess the significance of pathologically expressed Pn-ASVs as potential diagnostic and therapeutic targets. We found that most physiologically expressed Pn isoforms lacked exon 17 and 21. Next, we used human breast cancer and normal adjacent tissue (NAT) to investigate the expression pattern of Pn-ASVs under pathological conditions. Pn-ASVs with exon 21 were significantly increased in tumor tissues compared with NAT. In situ hybridization identified the synthesis of Pn-ASVs with exon 21 in peri-tumoral stromal cells. Additionally, the in vivo bio-distribution of 89Zr-labeled Pn antibody against exon 21 (Pn-21Ab) in mice bearing breast cancer demonstrated selective and specific accumulation in tumors, while Pn-21Ab significantly suppressed tumor growth in the mouse breast cancer model. (3) Conclusions: Together, these data indicate that Pn-ASVs might have potential for use as diagnostic and therapeutic targets for breast cancer.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98374
citation_title	Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer
citation_author	Kanemoto, Yuko
	Sanada, Fumihiro
	Shibata, Kana
	Tsunetoshi, Yasuo
	Katsuragi, Naruto
	Koibuchi, Nobutaka
	Yoshinami, Tetsuhiro
	Yamamoto, Koichi
	Morishita, Ryuichi
	Taniyama, Yoshiaki
	Shimazu, Kenzo
citation_publisher	Multidisciplinary Digital Publishing Institute (MDPI)
citation_language	English
citation_date	2024-09-01
citation_journal_title	Biomolecules
citation_volume	14
citation_issue	9
citation_firstpage	1093
citation_doi	https://doi.org/10.3390/biom14091093
citation_public_url	https://hdl.handle.net/11094/98374

Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer

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