Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging

Nagano, Erika; Odake, Kazuki; Shimma, Shuichi

Analytical and Bioanalytical Chemistry, 2024, 416(28), 6601-6610

Number of Access:273（2025-10-09 08:00 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98390

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Title
Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging
Creator
CreatorName
Nagano, Erika
CreatorName
Odake, Kazuki
NRID
1000030515896 1000030515896
researchmap permalink
shimmax shimmax
Scopus Author ID
23486936900 23486936900
CreatorName
Shimma, Shuichi
Subject
3-Nitrophenylhydrazine
Lactate
Mass spectrometry imaging
On-tissue chemical derivatization
Pyruvate
Description
The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05559-4.
Abstract
Pyruvate and lactate are the final metabolites of the glycolytic system that are formed under oxygen-rich and anaerobic conditions, respectively. They play an important role in energy metabolism. Obtaining a tissue distribution image of pyruvate and lactate holds great significance in molecular biology because the glycolytic system plays an essential role in diseases, such as tumors and diabetes; microbial activities, such as alcohol production and lactic acid fermentation; and maintaining homeostasis in the gut environment. However, it is difficult to obtain images of the distribution of in vivo metabolites because of the low detection sensitivities of current methods. In this study, a novel derivatization method for pyruvate and lactate was developed using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to detect pyruvate and lactate in vivo and obtain biodistribution images. We investigated derivatization methods using readily available 3-nitrophenylhydrazine (3NPH), the addition of which improves the sensitivity of pyruvate detection, and the distribution of pyruvate in mouse testes was successfully visualized. Furthermore, the distribution of lactate in the mouse testes could be visualized, and improved detection sensitivity for the main metabolites of the tricarboxylic acid cycle was demonstrated. This derivatization method can be used to detect carboxyl-containing metabolites, including pyruvate, via MALDI-MSI. Furthermore, 3NPH forms amide bonds with carbonyl, phosphate, and carboxyl groups, suggesting the possibility of visualizing its distribution in many metabolites.
Publisher
Springer Science and Business Media Deutschland GmbH
Source Title
Analytical and Bioanalytical Chemistry
Volume (Issue)
416(28)
Page
6601-6610
Date of Issued
2024-10-08
Language
English
Handle URL
https://hdl.handle.net/11094/98390
PISSN
16182642
eISSN
16182650
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s00216-024-05559-4
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article