Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma

Yamamoto, Akinaru; Kawashima, Atsunari; Sakai, Shinsuke; Mita, Masashi; Sassi, Nesrine; Inoguchi, Shunsuke; Horibe, Yuki; Yoshimura, Akihiro; Tani, Masaru; Yutong, Liu; Okuda, Yohei; Oka, Toshiki; Uemura, Toshihiro; Yamamichi, Gaku; Ishizuya, Yu; Hayashi, Takuji; Yamamoto, Yoshiyuki; Kato, Taigo; Hatano, Koji; Kakuta, Yoichi; Imamura, Ryoichi; Takahara, Shiro; Kimura, Tomonori; Nonomura, Norio

doi:https://doi.org/10.1016/j.bbrc.2024.150701

Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma

Yamamoto, Akinaru; Kawashima, Atsunari; Sakai, Shinsuke et al.

Biochemical and Biophysical Research Communications, 2024, 733, 150701

Number of Access:3,656（2025-10-12 12:23 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98435

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Title	Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma
Creator	NRID 1000050909653 1000050909653 researchmap permalink ayamamoto ayamamoto ORCID 0000-0002-3565-5870 0000-0002-3565-5870 CreatorName Yamamoto, Akinaru
	NRID 1000050746568 1000050746568 researchmap permalink akawashima akawashima Scopus Author ID 9846139400 9846139400 CreatorName Kawashima, Atsunari
	NRID 1000060817360 1000060817360 CreatorName Sakai, Shinsuke
	CreatorName Mita, Masashi
	CreatorName Sassi, Nesrine
	CreatorName Inoguchi, Shunsuke
	NRID 1000080981977 1000080981977 CreatorName Horibe, Yuki
	NRID 1000070981992 1000070981992 CreatorName Yoshimura, Akihiro
	NRID 1000090981960 1000090981960 CreatorName Tani, Masaru
	CreatorName Yutong, Liu
	NRID 1000010964372 1000010964372 ORCID 0000-0001-9909-4200 0000-0001-9909-4200 researchmap permalink okudayohei okudayohei CreatorName Okuda, Yohei
	NRID 1000060964385 1000060964385 CreatorName Oka, Toshiki
	NRID 1000060905941 1000060905941 CreatorName Uemura, Toshihiro
	NRID 1000040882262 1000040882262 researchmap permalink urology-osaka-japan urology-osaka-japan CreatorName Yamamichi, Gaku
	NRID 1000000783854 1000000783854 researchmap permalink okiishi okiishi CreatorName Ishizuya, Yu
	NRID 1000050747079 1000050747079 researchmap permalink takuhaya takuhaya CreatorName Hayashi, Takuji
	NRID 1000090759557 1000090759557 researchmap permalink yoshi229 yoshi229 ORCID 0000-0002-1271-3521 0000-0002-1271-3521 CreatorName Yamamoto, Yoshiyuki
	NRID 1000070648021 1000070648021 researchmap permalink taigokato taigokato Scopus Author ID 55414697500 55414697500 ORCID 0000-0002-8681-1407 0000-0002-8681-1407 CreatorName Kato, Taigo
	NRID 1000060762234 1000060762234 researchmap permalink koji_hatano koji_hatano Scopus Author ID 14627167600 14627167600 CreatorName Hatano, Koji
	NRID 1000040710116 1000040710116 researchmap permalink 987654321 987654321 ORCID 0000-0001-5339-4312 0000-0001-5339-4312 Scopus Author ID 23009020800 23009020800 CreatorName Kakuta, Yoichi
	NRID 1000040456976 1000040456976 CreatorName Imamura, Ryoichi
	NRID 1000070179547 1000070179547 researchmap permalink read0080308 read0080308 CreatorName Takahara, Shiro
	NRID 1000000631300 1000000631300 ORCID 0000-0001-8186-094X 0000-0001-8186-094X researchmap permalink KimuraUNM KimuraUNM Scopus Author ID 57199363473 57199363473 CreatorName Kimura, Tomonori
	NRID 1000030263263 1000030263263 researchmap permalink read0185096 read0185096 Scopus Author ID 7005880662 7005880662 CreatorName Nonomura, Norio
Subject	Biomarker
	Bladder cancer
	D-amino acid
	D-asparagine
	Liquid biopsy
	Urothelial carcinoma
Description	Yamamoto A., Kawashima A., Sakai S., et al. Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma. Biochemical and Biophysical Research Communications 733, 150701 (2024); https://doi.org/10.1016/j.bbrc.2024.150701.
Abstract	The sensitivity of currently available screening tools for urothelial carcinoma (UC) remains unsatisfactory particularly at early stages. Hence, we aimed to establish a novel blood-based screening tool for urothelial carcinoma. We measured serum D-amino acid levels in 108 and 192 patients with and without UC individuals in the derivation cohort, and 15 and 25 patients with and without UC in the validation cohort. Serum D-asparagine levels were significantly higher in patients with UC than in those without UC (p < 0.0001). We developed a novel screening equation for the diagnosis of urothelial carcinoma using D-asparagine in serum and estimated the glomerular filtration rate (eGFR). Serum D-asparagine levels adjusted for eGFR exhibited high performance in the diagnosis of UC (AUC-ROC, 0.869; sensitivity, 80.6 %; specificity, 82.7 %), even in early-stage UC (AUC-ROC: 0.859, sensitivity: 83.3 %, specificity: 82.3 %), which were previously misdiagnosed via urinary occult blood or urine cytology. This established strategy combined with urinary occult blood, improves diagnostic ability (sensitivity: 93.7 %, specificity: 70.1 %).
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	733
Page	150701
Date of Issued	2024-11-12
Language	English
Handle URL	https://hdl.handle.net/11094/98435
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2024.150701
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2024, 733, p. 150701
DC.title	Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma
DC.creator	Yamamoto, Akinaru
	Kawashima, Atsunari
	Sakai, Shinsuke
	Mita, Masashi
	Sassi, Nesrine
	Inoguchi, Shunsuke
	Horibe, Yuki
	Yoshimura, Akihiro
	Tani, Masaru
	Yutong, Liu
	Okuda, Yohei
	Oka, Toshiki
	Uemura, Toshihiro
	Yamamichi, Gaku
	Ishizuya, Yu
	Hayashi, Takuji
	Yamamoto, Yoshiyuki
	Kato, Taigo
	Hatano, Koji
	Kakuta, Yoichi
	Imamura, Ryoichi
	Takahara, Shiro
	Kimura, Tomonori
	Nonomura, Norio
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-12
DC.identifier	https://doi.org/10.1016/j.bbrc.2024.150701
DC.subject	Biomarker
	Bladder cancer
	D-amino acid
	D-asparagine
	Liquid biopsy
	Urothelial carcinoma
DC.description	Yamamoto A., Kawashima A., Sakai S., et al. Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma. Biochemical and Biophysical Research Communications 733, 150701 (2024); https://doi.org/10.1016/j.bbrc.2024.150701.
DCTERMS.abstract	The sensitivity of currently available screening tools for urothelial carcinoma (UC) remains unsatisfactory particularly at early stages. Hence, we aimed to establish a novel blood-based screening tool for urothelial carcinoma. We measured serum D-amino acid levels in 108 and 192 patients with and without UC individuals in the derivation cohort, and 15 and 25 patients with and without UC in the validation cohort. Serum D-asparagine levels were significantly higher in patients with UC than in those without UC (p < 0.0001). We developed a novel screening equation for the diagnosis of urothelial carcinoma using D-asparagine in serum and estimated the glomerular filtration rate (eGFR). Serum D-asparagine levels adjusted for eGFR exhibited high performance in the diagnosis of UC (AUC-ROC, 0.869; sensitivity, 80.6 %; specificity, 82.7 %), even in early-stage UC (AUC-ROC: 0.859, sensitivity: 83.3 %, specificity: 82.3 %), which were previously misdiagnosed via urinary occult blood or urine cytology. This established strategy combined with urinary occult blood, improves diagnostic ability (sensitivity: 93.7 %, specificity: 70.1 %).
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98435
citation_title	Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma
citation_author	Yamamoto, Akinaru
	Kawashima, Atsunari
	Sakai, Shinsuke
	Mita, Masashi
	Sassi, Nesrine
	Inoguchi, Shunsuke
	Horibe, Yuki
	Yoshimura, Akihiro
	Tani, Masaru
	Yutong, Liu
	Okuda, Yohei
	Oka, Toshiki
	Uemura, Toshihiro
	Yamamichi, Gaku
	Ishizuya, Yu
	Hayashi, Takuji
	Yamamoto, Yoshiyuki
	Kato, Taigo
	Hatano, Koji
	Kakuta, Yoichi
	Imamura, Ryoichi
	Takahara, Shiro
	Kimura, Tomonori
	Nonomura, Norio
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2024-11-12
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	733
citation_firstpage	150701
citation_doi	https://doi.org/10.1016/j.bbrc.2024.150701
citation_public_url	https://hdl.handle.net/11094/98435

Serum D-asparagine concentration adjusted for eGFR could serve as a novel screening tool for urothelial carcinoma

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