Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

Kusuma, Subhan Hadi; Kakizuka, Taishi; Hattori, Mitsuru et al.

Proceedings of the National Academy of Sciences of the United States of America, 2024, 121(41), e2406358121

Number of Access:595（2025-11-02 16:30 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98540

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Title
Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts
Creator
CreatorName
Kusuma, Subhan Hadi
ORCID
0000-0003-4269-5923 0000-0003-4269-5923
researchmap permalink
t-kakizuka t-kakizuka
CreatorName
Kakizuka, Taishi
NRID
1000020589858 1000020589858
researchmap permalink
mhatto mhatto
Scopus Author ID
7401882679 7401882679
ORCID
0000-0003-1189-6554 0000-0003-1189-6554
CreatorName
Hattori, Mitsuru
NRID
1000020311350 1000020311350
ORCID
0000-0003-2650-9895 0000-0003-2650-9895
researchmap permalink
ng1 ng1
Scopus Author ID
7403433377 7403433377
CreatorName
Nagai, Takeharu
Subject
bacterial luciferase
bioimaging
bioluminescence
biosensor
Description
Kusuma S.H., Kakizuka T., Hattori M., et al. Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts. Proceedings of the National Academy of Sciences of the United States of America 121, e2406358121 (2024); https://doi.org/10.1073/pnas.2406358121.
Abstract
Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five-color auto-bioluminescence system named Nano-lanternX (NLX), which can be expressed in bacterial, mammalian, and plant hosts, thereby enabling auto-bioluminescence in various living organisms. Utilizing spectral unmixing, we achieved the successful observation of multicolor auto-bioluminescence, enabling detailed single-cell imaging across both bacterial and mammalian cells. We have also expanded the applications of the NLX system, such as multiplexed auto-bioluminescence imaging for gene expression, protein localization, and dynamics of biomolecules within living mammalian cells.
Publisher
National Academy of Sciences
Source Title
Proceedings of the National Academy of Sciences of the United States of America
Volume (Issue)
121(41)
Page
e2406358121
Date of Issued
2024-10-08
Language
English
Handle URL
https://hdl.handle.net/11094/98540
PISSN
00278424
eISSN
10916490
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1073/pnas.2406358121
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article