Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer

Minamikawa, Takeo; Sakaguchi, Reiko; Harada, Yoshinori; Tanioka, Hiroki; Inoue, Sota; Hase, Hideharu; Mori, Yasuo; Takamatsu, Tetsuro; Yamasaki, Yu; Morimoto, Yukihiro; Kawasaki, Masahiro; Kawasaki, Mitsuo

doi:https://doi.org/10.1038/s41377-024-01655-3

Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer

Minamikawa, Takeo; Sakaguchi, Reiko; Harada, Yoshinori et al.

Light: Science and Applications, 2024, 13, 299

Number of Access:1,153（2025-10-26 04:21 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98543

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Title	Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer
Creator	NRID 1000010637193 1000010637193 ORCID 0000-0003-3528-7163 0000-0003-3528-7163 researchmap permalink takeominamikawa takeominamikawa CreatorName Minamikawa, Takeo
	CreatorName Sakaguchi, Reiko
	NRID 1000010381956 1000010381956 CreatorName Harada, Yoshinori
	CreatorName Tanioka, Hiroki
	CreatorName Inoue, Sota
	CreatorName Hase, Hideharu
	NRID 1000080212265 1000080212265 CreatorName Mori, Yasuo
	NRID 1000040154900 1000040154900 CreatorName Takamatsu, Tetsuro
	CreatorName Yamasaki, Yu
	NRID 1000080607218 1000080607218 researchmap permalink morimoto07218 morimoto07218 CreatorName Morimoto, Yukihiro
	NRID 1000070110723 1000070110723 CreatorName Kawasaki, Masahiro
	NRID 1000050111927 1000050111927 CreatorName Kawasaki, Mitsuo
Description	Minamikawa T., Sakaguchi R., Harada Y., et al. Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer. Light: Science and Applications 13, 299 (2024); https://doi.org/10.1038/s41377-024-01655-3.
Abstract	We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands (AgNIs) coated with column-structured silica (CSS) overlayer of over 100 nm thickness, namely, remote plasmonic-like enhancement (RPE). The CSS layer provides physical and chemical protection, reducing the impact between analyte molecules and metal nanostructures. RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(I)/halide solution. The RPE plate significantly enhances Raman scattering and fluorescence, even without proximity between analyte molecules and metal nanostructures. The maximum enhancement factors are 107-fold for Raman scattering and 102-fold for fluorescence. RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues. Our findings present an interesting deviation from the conventional near-field enhancement theory, as they cannot be readily explained within its framework. However, based on the phenomenological aspects we have demonstrated, the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte, facilitated through the CSS structure. Although further investigation is warranted to fully understand the underlying mechanisms, the RPE plate offers practical advantages, such as high productivity and biocompatibility, making it a valuable tool for biosensing and biomolecular analysis in chemistry, biology, and medicine. We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.
Publisher	Springer Nature
Source Title	Light: Science and Applications
Volume (Issue)	13
Page	299
Date of Issued	2024-10-28
Language	English
Handle URL	https://hdl.handle.net/11094/98543
PISSN	20955545
eISSN	20477538
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41377-024-01655-3
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Light: Science and Applications. 2024, 13, p. 299
DC.title	Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer
DC.creator	Minamikawa, Takeo
	Sakaguchi, Reiko
	Harada, Yoshinori
	Tanioka, Hiroki
	Inoue, Sota
	Hase, Hideharu
	Mori, Yasuo
	Takamatsu, Tetsuro
	Yamasaki, Yu
	Morimoto, Yukihiro
	Kawasaki, Masahiro
	Kawasaki, Mitsuo
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-10-28
DC.identifier	https://doi.org/10.1038/s41377-024-01655-3
DC.description	Minamikawa T., Sakaguchi R., Harada Y., et al. Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer. Light: Science and Applications 13, 299 (2024); https://doi.org/10.1038/s41377-024-01655-3.
DCTERMS.abstract	We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands (AgNIs) coated with column-structured silica (CSS) overlayer of over 100 nm thickness, namely, remote plasmonic-like enhancement (RPE). The CSS layer provides physical and chemical protection, reducing the impact between analyte molecules and metal nanostructures. RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(I)/halide solution. The RPE plate significantly enhances Raman scattering and fluorescence, even without proximity between analyte molecules and metal nanostructures. The maximum enhancement factors are 107-fold for Raman scattering and 102-fold for fluorescence. RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues. Our findings present an interesting deviation from the conventional near-field enhancement theory, as they cannot be readily explained within its framework. However, based on the phenomenological aspects we have demonstrated, the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte, facilitated through the CSS structure. Although further investigation is warranted to fully understand the underlying mechanisms, the RPE plate offers practical advantages, such as high productivity and biocompatibility, making it a valuable tool for biosensing and biomolecular analysis in chemistry, biology, and medicine. We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98543
citation_title	Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer
citation_author	Minamikawa, Takeo
	Sakaguchi, Reiko
	Harada, Yoshinori
	Tanioka, Hiroki
	Inoue, Sota
	Hase, Hideharu
	Mori, Yasuo
	Takamatsu, Tetsuro
	Yamasaki, Yu
	Morimoto, Yukihiro
	Kawasaki, Masahiro
	Kawasaki, Mitsuo
citation_publisher	Springer Nature
citation_language	English
citation_date	2024-10-28
citation_journal_title	Light: Science and Applications
citation_volume	13
citation_firstpage	299
citation_doi	https://doi.org/10.1038/s41377-024-01655-3
citation_public_url	https://hdl.handle.net/11094/98543

Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer

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