The SARS-CoV-2 ORF6 protein inhibits nuclear export of mRNA and spliceosomal U snRNA

Taniguchi, Ichiro

PLoS ONE, 2024, 19(10), e0312098

Number of Access:208（2026-07-18 22:55 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98544

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Title
The SARS-CoV-2 ORF6 protein inhibits nuclear export of mRNA and spliceosomal U snRNA
Creator
NRID
1000000467432 1000000467432
ORCID
0000-0002-9364-2680 0000-0002-9364-2680
researchmap permalink
00467432 00467432
CreatorName
Taniguchi, Ichiro
Description
Taniguchi I. (2024) The SARS-CoV-2 ORF6 protein inhibits nuclear export of mRNA and spliceosomal U snRNA. PLoS ONE 19(10): e0312098. doi:10.1371/journal.pone.0312098.
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 19 (COVID-19). SARS-CoV-2 infection suppresses host innate immunity and impairs cell viability. Among the viral proteins, ORF6 exhibits potent interferon (IFN) antagonistic activity and cellular toxicity. It also interacts with the RNA export factor RAE1, which bridges the nuclear pore complex and nuclear export receptors, suggesting an effect on RNA export. Using the Xenopus oocyte microinjection system, I found that ORF6 blocked the export of not only mRNA but also spliceosomal U snRNA. I further demonstrated that ORF6 affects the interaction between RAE1 and nuclear export receptors and inhibits the RNA binding of RAE1. These effects of ORF6 may cumulatively block the export of several classes of RNA. I also found that ORF6 binds RNA and forms oligomers. These findings provide insights into the suppression of innate immune responses and the reduction in cell viability caused by SARS-CoV-2 infection, contributing to the development of antiviral drugs targeting ORF6.
Publisher
Public Library of Science
Source Title
PLoS ONE
Volume (Issue)
19(10)
Page
e0312098
Date of Issued
2024-10-31
Language
English
Handle URL
https://hdl.handle.net/11094/98544
eISSN
19326203
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DOI (Publisher Version)
https://doi.org/10.1371/journal.pone.0312098
Access Rights
open access
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This article is licensed under a Creative Commons Attribution 4.0 International License.
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Version of Record
Category
学術雑誌論文 Journal Article