Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer

Hamada, Masakazu; Nishiyama, Kyoko; Nomura, Ryota; Akitomo, Tatsuya; Mitsuhata, Chieko; Yura, Yoshiaki; Nakano, Kazuhiko; Matsumoto-Nakano, Michiyo; Uzawa, Narikazu; Inaba, Hiroaki

doi:https://doi.org/10.1016/j.heliyon.2024.e39284

Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer

Hamada, Masakazu; Nishiyama, Kyoko; Nomura, Ryota et al.

Heliyon, 2024, 10(20), e39284

Number of Access:1,073（2025-07-20 11:42 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98547

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Title	Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer
Creator	NRID 1000080506361 1000080506361 researchmap permalink 6361 6361 Scopus Author ID 36616148000 36616148000 CreatorName Hamada, Masakazu
	NRID 1000070846542 1000070846542 researchmap permalink 02205 02205 CreatorName Nishiyama, Kyoko
	NRID 1000090437385 1000090437385 CreatorName Nomura, Ryota
	NRID 1000090909681 1000090909681 CreatorName Akitomo, Tatsuya
	NRID 1000010335664 1000010335664 CreatorName Mitsuhata, Chieko
	NRID 1000000136277 1000000136277 CreatorName Yura, Yoshiaki
	NRID 1000000379083 1000000379083 researchmap permalink read0202427 read0202427 Scopus Author ID 7402011667 7402011667 CreatorName Nakano, Kazuhiko
	NRID 1000030359848 1000030359848 CreatorName Matsumoto-Nakano, Michiyo
	NRID 1000030345285 1000030345285 researchmap permalink read0212957 read0212957 Scopus Author ID 6603144046 6603144046 CreatorName Uzawa, Narikazu
	NRID 1000070359850 1000070359850 CreatorName Inaba, Hiroaki
Subject	Fusobacterium
	Head and neck cancer
	Intratumor microbiome
	Oral bacteria
	Prevotella
	RNA sequencing
	Streptococcus
	TCGA
	TCMA
Description	Hamada M., Nishiyama K., Nomura R., et al. Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer. Heliyon 10, e39284 (2024); https://doi.org/10.1016/j.heliyon.2024.e39284.
Abstract	A bioinformatic analysis is a promising approach to understand the relationship between the vast tumor microbiome and cancer development. In the present study, we studied the relationships between the intratumoral microbiome and classical clinical risk factors using bioinformatics analysis of the Cancer Genome Atlas (TCGA) and the Cancer Microbiome Atlas (TCMA) datasets. We used TCMA database and investigated the abundance of microbes at the genus level in solid normal tissue (n = 22) and the primary tumors of patients with head and neck squamous cell carcinoma (HNSCC) (n = 154) and identified three major tumor microbiomes, Fusobacterium, Prevotella, and Streptococcus. The tissue level of Fusobacterium was higher in primary tumors than in solid normal tissue. However, univariate and multivariate analyses of these 3 microbes showed no significant effects on patient survival. We then extracted 43, 55, or 59 genes that were differentially expressed between the over and under the median groups for Fusobacterium, Prevotella, or Streptococcus using the criteria of >2.5, >1.5, or >2.0 fold and p < 0.05 in the Mann-Whitney U test. The results of a pathway analysis revealed the association of Fusobacterium- and Streptococcus-related genes with the IL-17 signaling pathway and Staphylococcus aureus infection, while Prevotella-associated pathways were not extracted. A protein-protein interaction analysis revealed a dense network in the order of Fusobacterium, Streptococcus, and Prevotella. An investigation of the relationships between the intratumoral microbiome and classical clinical risk factors showed that high levels of Fusobacterium were associated with a good prognosis in the absence of alcohol consumption and smoking, while high levels of Streptococcus were associated with a poor prognosis in the absence of alcohol consumption. In conclusion, intratumoral Fusobacterium and Streptococcus may affect the prognosis of patients with HNSCC, and their effects on HNSCC are modulated by the impact of drinking and smoking.
Publisher	Elsevier Ltd
Source Title	Heliyon
Volume (Issue)	10(20)
Page	e39284
Date of Issued	2024-10-30
Language	English
Handle URL	https://hdl.handle.net/11094/98547
eISSN	24058440
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.heliyon.2024.e39284
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Heliyon. 2024, 10(20), p. e39284
DC.title	Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer
DC.creator	Hamada, Masakazu
	Nishiyama, Kyoko
	Nomura, Ryota
	Akitomo, Tatsuya
	Mitsuhata, Chieko
	Yura, Yoshiaki
	Nakano, Kazuhiko
	Matsumoto-Nakano, Michiyo
	Uzawa, Narikazu
	Inaba, Hiroaki
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-10-30
DC.identifier	https://doi.org/10.1016/j.heliyon.2024.e39284
DC.subject	Fusobacterium
	Head and neck cancer
	Intratumor microbiome
	Oral bacteria
	Prevotella
	RNA sequencing
	Streptococcus
	TCGA
	TCMA
DC.description	Hamada M., Nishiyama K., Nomura R., et al. Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer. Heliyon 10, e39284 (2024); https://doi.org/10.1016/j.heliyon.2024.e39284.
DCTERMS.abstract	A bioinformatic analysis is a promising approach to understand the relationship between the vast tumor microbiome and cancer development. In the present study, we studied the relationships between the intratumoral microbiome and classical clinical risk factors using bioinformatics analysis of the Cancer Genome Atlas (TCGA) and the Cancer Microbiome Atlas (TCMA) datasets. We used TCMA database and investigated the abundance of microbes at the genus level in solid normal tissue (n = 22) and the primary tumors of patients with head and neck squamous cell carcinoma (HNSCC) (n = 154) and identified three major tumor microbiomes, Fusobacterium, Prevotella, and Streptococcus. The tissue level of Fusobacterium was higher in primary tumors than in solid normal tissue. However, univariate and multivariate analyses of these 3 microbes showed no significant effects on patient survival. We then extracted 43, 55, or 59 genes that were differentially expressed between the over and under the median groups for Fusobacterium, Prevotella, or Streptococcus using the criteria of >2.5, >1.5, or >2.0 fold and p < 0.05 in the Mann-Whitney U test. The results of a pathway analysis revealed the association of Fusobacterium- and Streptococcus-related genes with the IL-17 signaling pathway and Staphylococcus aureus infection, while Prevotella-associated pathways were not extracted. A protein-protein interaction analysis revealed a dense network in the order of Fusobacterium, Streptococcus, and Prevotella. An investigation of the relationships between the intratumoral microbiome and classical clinical risk factors showed that high levels of Fusobacterium were associated with a good prognosis in the absence of alcohol consumption and smoking, while high levels of Streptococcus were associated with a poor prognosis in the absence of alcohol consumption. In conclusion, intratumoral Fusobacterium and Streptococcus may affect the prognosis of patients with HNSCC, and their effects on HNSCC are modulated by the impact of drinking and smoking.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	98547
citation_title	Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer
citation_author	Hamada, Masakazu
	Nishiyama, Kyoko
	Nomura, Ryota
	Akitomo, Tatsuya
	Mitsuhata, Chieko
	Yura, Yoshiaki
	Nakano, Kazuhiko
	Matsumoto-Nakano, Michiyo
	Uzawa, Narikazu
	Inaba, Hiroaki
citation_publisher	Elsevier Ltd
citation_language	English
citation_date	2024-10-30
citation_journal_title	Heliyon
citation_volume	10
citation_issue	20
citation_firstpage	e39284
citation_doi	https://doi.org/10.1016/j.heliyon.2024.e39284
citation_public_url	https://hdl.handle.net/11094/98547

Clinical relationships between the intratumoral microbiome and risk factors for head and neck cancer

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