Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies

Ikuta, Shoko; Saito, Yutaka; Takata, So et al.

Molecular Cancer, 2024, 23, 249

Number of Access:1,483（2025-08-14 03:30 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98555

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
MolCancer_23_249 pdf None 4.02 MB 41 2024.12.02  
Creative Commons : Attribution - NonCommercial - NoDerivatives

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies
Creator
CreatorName
Ikuta, Shoko
NRID
1000090501859 1000090501859
CreatorName
Saito, Yutaka
CreatorName
Takata, So
NRID
1000010847891 1000010847891
ORCID
0000-0001-7604-0242 0000-0001-7604-0242
researchmap permalink
nakatani_yoichiro nakatani_yoichiro
Scopus Author ID
8534216500 8534216500
CreatorName
Nakatani, Yoichiro
NRID
1000010570583 1000010570583
researchmap permalink
saxon-w saxon-w
Scopus Author ID
13404305500 13404305500
CreatorName
Nagatomo, Izumi
NRID
1000000797003 1000000797003
CreatorName
Shiba, Satoshi
NRID
1000040452388 1000040452388
researchmap permalink
yoshi10 yoshi10
Scopus Author ID
7404001848 7404001848
CreatorName
Takeda, Yoshito
NRID
1000010373333 1000010373333
researchmap permalink
ytotoki ytotoki
Scopus Author ID
58966284600 58966284600
CreatorName
Totoki, Yasushi
NRID
1000070790278 1000070790278
CreatorName
Mizutani, Sayaka
NRID
1000050844792 1000050844792
CreatorName
Sunakawa, Hironori
CreatorName
Ikematsu, Hiroaki
CreatorName
Takamaru, Hiroyuki
NRID
1000010294125 1000010294125
researchmap permalink
read0051725 read0051725
Scopus Author ID
6602726311 6602726311
ORCID
0000-0003-4749-7117 0000-0003-4749-7117
CreatorName
Kumanogoh, Atsushi
NRID
1000020359920 1000020359920
researchmap permalink
read0210591 read0210591
Scopus Author ID
57226203270 57226203270
CreatorName
Yachida, Shinichi
Subject
Adenoma
CIBERSORTx
Colorectal cancer
Field cancerization
Immune cell
Left-sided colorectum
Right-sided colon
Terminal ileum
Transcriptome
Description
Ikuta S., Saito Y., Takata S., et al. Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies. Molecular Cancer 23, 249 (2024); https://doi.org/10.1186/s12943-024-02159-9.
Abstract
A comprehensive endoscopic small and large intestinal untargeted step biopsy procedure was conducted to compare gene expression between the normal intestinal mucosa of healthy individuals and that of patients with colorectal tumors. From 78 participants (healthy individuals [n = 17], patients with colorectal conventional adenomas [n = 6], patients with Tis–T1 colorectal cancer [n = 41], patients with T2–4 colorectal cancer [n = 14]), biopsies of normal mucosa of the terminal ileum, right-sided colon (cecum and ascending colon), and left-sided colorectum (descending colon, sigmoid colon, and rectum) were obtained using a lower gastrointestinal endoscope. RNA was extracted from all samples, and total transcriptome sequencing was performed. Transcriptome data from 388 samples was analyzed. DNA was also extracted from tumor biopsy tissues and analyzed for whole-exome sequencing. In healthy individuals, gene expression differed significantly among the terminal ileum, right-sided colon, and left-sided colorectum, presumably linked to embryological factors. There were differences in gene expression in the normal mucosa in colorectal cancer patients, compared to healthy controls. Patients with tumors, especially T2–4 colorectal cancer, showed considerable variation in gene expression in non-tumor tissues, even in the terminal ileum distant from the tumor site. Based on endoscopic biopsies, the results imply cancer-predisposing conditions in seemingly normal tissues. The present study points to the importance of small intestine and cancer-predisposing conditions in the colon of colorectal cancer patients, with possible implications for developing novel immunotherapy and other therapeutic modalities.
Publisher
BioMed Central Ltd
Source Title
Molecular Cancer
Volume (Issue)
23
Page
249
Date of Issued
2024-11-07
Language
English
Handle URL
https://hdl.handle.net/11094/98555
eISSN
14764598
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1186/s12943-024-02159-9
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article