Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis

Fukuda, Masatoshi; Okanishi, Hiroki; Ino, Daisuke; Ono, Kazuya; Ota, Takeru; Wakai, Eri; Sato, Takashi; Ohta, Yumi; Kikkawa, Yoshiaki; Inohara, Hidenori; Kanai, Yoshikatsu; Hibino, Hiroshi

doi:https://doi.org/10.1016/j.isci.2024.111214

Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis

Fukuda, Masatoshi; Okanishi, Hiroki; Ino, Daisuke et al.

iScience, 2024, 27(11), 111214

Number of Access:1,277（2025-06-29 13:37 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98558

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Title	Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis
Creator	NRID 1000060909638 1000060909638 CreatorName Fukuda, Masatoshi
	NRID 1000070792589 1000070792589 researchmap permalink 7000021427 7000021427 Scopus Author ID 56593651700 56593651700 CreatorName Okanishi, Hiroki
	NRID 1000040721981 1000040721981 ORCID 0000-0002-8112-0746 0000-0002-8112-0746 researchmap permalink daisukeino daisukeino CreatorName Ino, Daisuke
	NRID 1000070972165 1000070972165 ORCID 0000-0002-3857-0055 0000-0002-3857-0055 researchmap permalink drono drono CreatorName Ono, Kazuya
	NRID 1000030790571 1000030790571 ORCID 0000-0003-0011-8925 0000-0003-0011-8925 researchmap permalink otatakeru otatakeru Scopus Author ID 26642091900 26642091900 CreatorName Ota, Takeru
	NRID 1000040964896 1000040964896 researchmap permalink eri_wakai eri_wakai CreatorName Wakai, Eri
	NRID 1000030756002 1000030756002 researchmap permalink 4501 4501 Scopus Author ID 7406982585 7406982585 CreatorName Sato, Takashi
	NRID 1000000598401 1000000598401 researchmap permalink osaka-ent osaka-ent Scopus Author ID 54416193500 54416193500 CreatorName Ohta, Yumi
	NRID 1000020280787 1000020280787 CreatorName Kikkawa, Yoshiaki
	NRID 1000000273657 1000000273657 researchmap permalink read0046473 read0046473 Scopus Author ID 7004501837 7004501837 CreatorName Inohara, Hidenori
	NRID 1000060204533 1000060204533 researchmap permalink read0063550 read0063550 Scopus Author ID 55575124400 55575124400 CreatorName Kanai, Yoshikatsu
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 CreatorName Hibino, Hiroshi
Subject	Biochemical analysis
	Molecular physiology
	Proteomics
Description	Fukuda M., Okanishi H., Ino D., et al. Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis. iScience 27, 111214 (2024); https://doi.org/10.1016/j.isci.2024.111214.
Abstract	The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α2-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α2-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.
Publisher	Elsevier Inc.
Source Title	iScience
Volume (Issue)	27(11)
Page	111214
Date of Issued	2024-11-15
Language	English
Handle URL	https://hdl.handle.net/11094/98558
eISSN	25890042
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.isci.2024.111214
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	iScience. 2024, 27(11), p. 111214
DC.title	Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis
DC.creator	Fukuda, Masatoshi
	Okanishi, Hiroki
	Ino, Daisuke
	Ono, Kazuya
	Ota, Takeru
	Wakai, Eri
	Sato, Takashi
	Ohta, Yumi
	Kikkawa, Yoshiaki
	Inohara, Hidenori
	Kanai, Yoshikatsu
	Hibino, Hiroshi
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-15
DC.identifier	https://doi.org/10.1016/j.isci.2024.111214
DC.subject	Biochemical analysis
	Molecular physiology
	Proteomics
DC.description	Fukuda M., Okanishi H., Ino D., et al. Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis. iScience 27, 111214 (2024); https://doi.org/10.1016/j.isci.2024.111214.
DCTERMS.abstract	The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α2-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α2-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98558
citation_title	Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis
citation_author	Fukuda, Masatoshi
	Okanishi, Hiroki
	Ino, Daisuke
	Ono, Kazuya
	Ota, Takeru
	Wakai, Eri
	Sato, Takashi
	Ohta, Yumi
	Kikkawa, Yoshiaki
	Inohara, Hidenori
	Kanai, Yoshikatsu
	Hibino, Hiroshi
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2024-11-15
citation_journal_title	iScience
citation_volume	27
citation_issue	11
citation_firstpage	111214
citation_doi	https://doi.org/10.1016/j.isci.2024.111214
citation_public_url	https://hdl.handle.net/11094/98558

Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis

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