Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna

Shimizu, Rina; Sakamoto, Joe; Adhitama, Nikko; Fujikawa, Mana; Religia, Pijar; Kamei, Yasuhiro; Watanabe, Hajime; Kato, Yasuhiko

doi:https://doi.org/10.1038/s41598-024-77458-8

Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna

Shimizu, Rina; Sakamoto, Joe; Adhitama, Nikko et al.

Scientific Reports, 2024, 14, 25696

Number of Access:740（2025-10-09 08:16 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98560

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Title	Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna
Creator	CreatorName Shimizu, Rina
	NRID 1000080804145 1000080804145 CreatorName Sakamoto, Joe
	NRID 1000000905953 1000000905953 ORCID 0000-0001-7864-7654 0000-0001-7864-7654 researchmap permalink adhitamanikko adhitamanikko Scopus Author ID 57202986462 57202986462 CreatorName Adhitama, Nikko
	CreatorName Fujikawa, Mana
	ORCID 0000-0002-9349-2993 0000-0002-9349-2993 researchmap permalink pijar_religia pijar_religia CreatorName Religia, Pijar
	NRID 1000070372563 1000070372563 CreatorName Kamei, Yasuhiro
	NRID 1000080212322 1000080212322 ORCID 0000-0001-9657-6554 0000-0001-9657-6554 researchmap permalink read0052409 read0052409 Scopus Author ID 35466703500 35466703500 CreatorName Watanabe, Hajime
	NRID 1000030837050 1000030837050 researchmap permalink katoY katoY Scopus Author ID 55473605900 55473605900 CreatorName Kato, Yasuhiko
Description	Shimizu R., Sakamoto J., Adhitama N., et al. Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna. Scientific Reports 14, 25696 (2024); https://doi.org/10.1038/s41598-024-77458-8.
Abstract	The crustacean Daphnia magna is an emerging model for ecological and toxicological genomics. However, the lack of methods for spatial and temporal control of gene expression has impaired the elucidation of molecular mechanisms underlying responses to environments in vivo. Here we report local activation of the hsp70 promoter-driven gene cassette in D. magna by the infrared laser-evoked gene operator (IR-LEGO), a method for heating the target cells with infrared irradiation. We identified the heat-inducible promoter upstream of the D. magna hsp70-A gene. Using this promoter, we generated a transgenic Daphnia harboring the heat-shock responsive GFP reporter gene and confirmed that the GFP gene responds to heat treatment not only in juveniles and adults but also in embryos. We collected embryos from the reporter line and irradiated four different regions of interest in the embryos: a proximal region of the third thoracic segment, a part of the midline, a second maxilla, and a distal region of the endopodite of the second antenna, all of which increased GFP fluorescence with an infrared laser. Our results suggest that the IR-LEGO method is useful for spatial and temporal control of gene expression and would advance the functional genomics in D. magna.
Publisher	Nature Research
Source Title	Scientific Reports
Volume (Issue)	14
Page	25696
Date of Issued	2024-10-28
Language	English
Handle URL	https://hdl.handle.net/11094/98560
eISSN	20452322
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1038/s41598-024-77458-8
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Scientific Reports. 2024, 14, p. 25696
DC.title	Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna
DC.creator	Shimizu, Rina
	Sakamoto, Joe
	Adhitama, Nikko
	Fujikawa, Mana
	Religia, Pijar
	Kamei, Yasuhiro
	Watanabe, Hajime
	Kato, Yasuhiko
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-10-28
DC.identifier	https://doi.org/10.1038/s41598-024-77458-8
DC.description	Shimizu R., Sakamoto J., Adhitama N., et al. Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna. Scientific Reports 14, 25696 (2024); https://doi.org/10.1038/s41598-024-77458-8.
DCTERMS.abstract	The crustacean Daphnia magna is an emerging model for ecological and toxicological genomics. However, the lack of methods for spatial and temporal control of gene expression has impaired the elucidation of molecular mechanisms underlying responses to environments in vivo. Here we report local activation of the hsp70 promoter-driven gene cassette in D. magna by the infrared laser-evoked gene operator (IR-LEGO), a method for heating the target cells with infrared irradiation. We identified the heat-inducible promoter upstream of the D. magna hsp70-A gene. Using this promoter, we generated a transgenic Daphnia harboring the heat-shock responsive GFP reporter gene and confirmed that the GFP gene responds to heat treatment not only in juveniles and adults but also in embryos. We collected embryos from the reporter line and irradiated four different regions of interest in the embryos: a proximal region of the third thoracic segment, a part of the midline, a second maxilla, and a distal region of the endopodite of the second antenna, all of which increased GFP fluorescence with an infrared laser. Our results suggest that the IR-LEGO method is useful for spatial and temporal control of gene expression and would advance the functional genomics in D. magna.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98560
citation_title	Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna
citation_author	Shimizu, Rina
	Sakamoto, Joe
	Adhitama, Nikko
	Fujikawa, Mana
	Religia, Pijar
	Kamei, Yasuhiro
	Watanabe, Hajime
	Kato, Yasuhiko
citation_publisher	Nature Research
citation_language	English
citation_date	2024-10-28
citation_journal_title	Scientific Reports
citation_volume	14
citation_firstpage	25696
citation_doi	https://doi.org/10.1038/s41598-024-77458-8
citation_public_url	https://hdl.handle.net/11094/98560

Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna

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