Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells

Matsuzaki, Takahisa; Fujii, Mai; Noro, Hayata; Togo, Shodai; Watanabe, Mami; Suganuma, Masami; Sharma, Shivani; Kobayashi, Naritaka; Kawamura, Ryuzo; Nakabayashi, Seiichiro; Yoshikawa, Hiroshi Y.

doi:https://doi.org/10.1073/pnas.2412914121

Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells

Matsuzaki, Takahisa; Fujii, Mai; Noro, Hayata et al.

Biophysics and Computational Biology, 2024, 121(50), 867

Number of Access:1,350（2025-10-31 17:23 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98577

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Title	Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells
Creator	NRID 1000050830527 1000050830527 ORCID 0000-0001-9466-7495 0000-0001-9466-7495 researchmap permalink 7000026401 7000026401 Scopus Author ID 55317252000 55317252000 CreatorName Matsuzaki, Takahisa
	CreatorName Fujii, Mai
	CreatorName Noro, Hayata
	CreatorName Togo, Shodai
	CreatorName Watanabe, Mami
	NRID 1000020196695 1000020196695 CreatorName Suganuma, Masami
	CreatorName Sharma, Shivani
	NRID 1000040595998 1000040595998 CreatorName Kobayashi, Naritaka
	NRID 1000050534591 1000050534591 CreatorName Kawamura, Ryuzo
	NRID 1000070180346 1000070180346 CreatorName Nakabayashi, Seiichiro
	NRID 1000050551173 1000050551173 ORCID 0000-0003-0624-6039 0000-0003-0624-6039 researchmap permalink read0108058 read0108058 Scopus Author ID 55708129100 55708129100 CreatorName Yoshikawa, Hiroshi Y.
Description	Matsuzaki Takahisa, Fujii Mai, Noro Hayata, et al. Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells. Proceedings of the National Academy of Sciences 121, 867 (2024); https://doi.org/10.1073/pnas.2412914121.
Abstract	We developed an advanced optical microscope for the simultaneous visualization of membrane fluidity and morphology to define cell adhesion signatures. This microscope combines ratiometric spectral imaging of membrane fluidity and interferometric imaging of membrane morphology. As a preliminary demonstration, we simultaneously visualized the interface between a giant unilamellar vesicle (GUV) and a glass substrate at different temperatures. We identified more fluid regions of the membrane and membrane adhesion sites (conversely, low-fluidic, ordered membrane domains correlate with nonadhered regions). This microscopic system was applied to human breast cancer cell lines with different malignancies; then, we identified adhesion signature of cancer cells: 1) low-fluidic, ordered membrane domains at the cell periphery and 2) large fluidic deviation at the nonadhered region. Inhibition of the cholesterol synthesis pathway suppresses the ordered membrane domains at the cancer cell periphery; thus, high level of cholesterol supports the appearance. Furthermore, an inhibitor of the unsaturated lipid synthesis pathway suppressed the large fluidic deviation at the nonadhered region; variation of unsaturated lipids contributes to heterogeneity of the cancer membrane. Therefore, our advanced optical microscopy enables us to couple membrane physical properties with cell adhesion, leading to definition of adhesion signatures of broad cell types, not just for cancer cells, that regulate life phenomena.
Publisher	National Academy of Sciences
Source Title	Biophysics and Computational Biology
Volume (Issue)	121(50)
Page	867
Date of Issued	2024-12-10
Language	English
Handle URL	https://hdl.handle.net/11094/98577
PISSN	00278424
eISSN	10916490
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1073/pnas.2412914121
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biophysics and Computational Biology. 2024, 121(50), p. 867
DC.title	Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells
DC.creator	Matsuzaki, Takahisa
	Fujii, Mai
	Noro, Hayata
	Togo, Shodai
	Watanabe, Mami
	Suganuma, Masami
	Sharma, Shivani
	Kobayashi, Naritaka
	Kawamura, Ryuzo
	Nakabayashi, Seiichiro
	Yoshikawa, Hiroshi Y.
DC.publisher	National Academy of Sciences
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-12-10
DC.identifier	https://doi.org/10.1073/pnas.2412914121
DC.description	Matsuzaki Takahisa, Fujii Mai, Noro Hayata, et al. Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells. Proceedings of the National Academy of Sciences 121, 867 (2024); https://doi.org/10.1073/pnas.2412914121.
DCTERMS.abstract	We developed an advanced optical microscope for the simultaneous visualization of membrane fluidity and morphology to define cell adhesion signatures. This microscope combines ratiometric spectral imaging of membrane fluidity and interferometric imaging of membrane morphology. As a preliminary demonstration, we simultaneously visualized the interface between a giant unilamellar vesicle (GUV) and a glass substrate at different temperatures. We identified more fluid regions of the membrane and membrane adhesion sites (conversely, low-fluidic, ordered membrane domains correlate with nonadhered regions). This microscopic system was applied to human breast cancer cell lines with different malignancies; then, we identified adhesion signature of cancer cells: 1) low-fluidic, ordered membrane domains at the cell periphery and 2) large fluidic deviation at the nonadhered region. Inhibition of the cholesterol synthesis pathway suppresses the ordered membrane domains at the cancer cell periphery; thus, high level of cholesterol supports the appearance. Furthermore, an inhibitor of the unsaturated lipid synthesis pathway suppressed the large fluidic deviation at the nonadhered region; variation of unsaturated lipids contributes to heterogeneity of the cancer membrane. Therefore, our advanced optical microscopy enables us to couple membrane physical properties with cell adhesion, leading to definition of adhesion signatures of broad cell types, not just for cancer cells, that regulate life phenomena.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98577
citation_title	Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells
citation_author	Matsuzaki, Takahisa
	Fujii, Mai
	Noro, Hayata
	Togo, Shodai
	Watanabe, Mami
	Suganuma, Masami
	Sharma, Shivani
	Kobayashi, Naritaka
	Kawamura, Ryuzo
	Nakabayashi, Seiichiro
	Yoshikawa, Hiroshi Y.
citation_publisher	National Academy of Sciences
citation_language	English
citation_date	2024-12-10
citation_journal_title	Biophysics and Computational Biology
citation_volume	121
citation_issue	50
citation_firstpage	867
citation_doi	https://doi.org/10.1073/pnas.2412914121
citation_public_url	https://hdl.handle.net/11094/98577

Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells

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