Immobilization of laccases on mechanically ground silk fibroin nanofibers for enhanced stability

Miyawaki, Ayari; Sakai, Shinji

International Journal of Biological Macromolecules, 2024, 282(1), 136745

Number of Access:268（2025-09-17 08:09 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98598

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Title
Immobilization of laccases on mechanically ground silk fibroin nanofibers for enhanced stability
Creator
CreatorName
Miyawaki, Ayari
NRID
1000020359938 1000020359938
ORCID
0000-0002-1041-4798 0000-0002-1041-4798
researchmap permalink
sakaishinji sakaishinji
Scopus Author ID
35450082900 35450082900
CreatorName
Sakai, Shinji
Subject
Enzyme immobilization
Laccase
Silk fibroin nanofiber
Description
Miyawaki A., Sakai S. Immobilization of laccases on mechanically ground silk fibroin nanofibers for enhanced stability. International Journal of Biological Macromolecules 282, 136745 (2024); https://doi.org/10.1016/j.ijbiomac.2024.136745.
Abstract
Azo dyes in textile industry effluents pose significant health and environmental risks. Laccase is an enzyme capable of degrading azo dyes, offering an environmentally friendly solution for treating textile wastewater. However, laccases need to be immobilized on specific carriers to enable effective reuse in batch reactors and continuous operation in flow-through reactors. This study employed silk fibroin nanofibers (SFNFs) obtained by mechanically grinding degummed silkworm silk as sustainable carriers to immobilize laccases through carbodiimide-mediated crosslinking. The immobilized laccases (SFNF-laccases) exhibited improved pH tolerance in the range of pH 3.0–8.0 with a smaller reduction in activity compared to free laccases (SFNF-laccases: 32.9 %, free laccases: 50.4 %). The thermal stability of immobilized laccases was also improved, showing 19, 13, and 9 % higher activities than those of free laccases at 40, 50, and 60 °C, respectively. After 8 days of storage, the activity of SFNF-laccases was 79 % of their activity immediately after immobilization, whereas free laccases retained only 29 % of their initial activity. In addition, SFNF-laccases maintained 73 % of their original operational activity in a flow-through reactor after 8 days. These results demonstrate the great potential of mechanically ground SFNFs as carriers of laccase and the resulting SFNF-laccases in industrial wastewater treatment.
Publisher
Elsevier B.V.
Source Title
International Journal of Biological Macromolecules
Volume (Issue)
282(1)
Page
136745
Date of Issued
2024-12-01
Language
English
Handle URL
https://hdl.handle.net/11094/98598
PISSN
01418130
eISSN
18790003
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.ijbiomac.2024.136745
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article