Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity

Morimoto, Soyoko; Fujiki, Fumihiro; Kondo, Kenta; Nakajima, Hiroko; Kobayashi, Yoshiki; Inatome, Miki; Aoyama, Nao; Nishida, Yuya; Tsuboi, Akihiro; Oka, Yoshihiro; Nishida, Sumiyuki; Nakata, Jun; Hosen, Naoki; Oji, Yusuke; Sugiyama, Haruo

doi:https://doi.org/10.18632/oncotarget.26139

Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity

Morimoto, Soyoko; Fujiki, Fumihiro; Kondo, Kenta et al.

Oncotarget, 2018, 9(75), 34132-34141

Number of Access:2,296（2025-08-14 18:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98828

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
Oncotarget_9_75_34132	pdf	None	1.35 MB	26	2024.12.23

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity
Creator	NRID 1000010649023 1000010649023 ORCID 0000-0002-4939-0455 0000-0002-4939-0455 researchmap permalink so-yo-ko so-yo-ko Scopus Author ID 35083938700 35083938700 CreatorName Morimoto, Soyoko
	NRID 1000040456926 1000040456926 researchmap permalink handai-fufujiki handai-fufujiki Scopus Author ID 6603301225 6603301225 CreatorName Fujiki, Fumihiro
	NRID 1000060779974 1000060779974 CreatorName Kondo, Kenta
	researchmap permalink hero.nakajima hero.nakajima CreatorName Nakajima, Hiroko
	CreatorName Kobayashi, Yoshiki
	CreatorName Inatome, Miki
	CreatorName Aoyama, Nao
	NRID 1000010793440 1000010793440 ORCID 0000-0002-1994-0807 0000-0002-1994-0807 researchmap permalink y_nishida y_nishida CreatorName Nishida, Yuya
	NRID 1000010372608 1000010372608 researchmap permalink atsuboi atsuboi Scopus Author ID 7005581966 7005581966 CreatorName Tsuboi, Akihiro
	NRID 1000020273691 1000020273691 researchmap permalink read0046463 read0046463 CreatorName Oka, Yoshihiro
	NRID 1000000403189 1000000403189 researchmap permalink sumi_nishi_12 sumi_nishi_12 Scopus Author ID 55190147500 55190147500 CreatorName Nishida, Sumiyuki
	NRID 1000090528952 1000090528952 researchmap permalink mogura mogura Scopus Author ID 37066306900 37066306900 CreatorName Nakata, Jun
	NRID 1000010456923 1000010456923 researchmap permalink hnaoki hnaoki Scopus Author ID 55928728700 55928728700 ORCID 0000-0001-9570-0947 0000-0001-9570-0947 CreatorName Hosen, Naoki
	NRID 1000020294100 1000020294100 researchmap permalink OJIYusukeOsakaU1-7 OJIYusukeOsakaU1-7 Scopus Author ID 7003801749 7003801749 CreatorName Oji, Yusuke
	NRID 1000070162906 1000070162906 researchmap permalink read0191156 read0191156 Scopus Author ID 55479602000 55479602000 CreatorName Sugiyama, Haruo
Subject	TCR
	TCR functional avidity
	TCR-engineered T-cell therapy
	WT1
Description	Morimoto S., Fujiki F., Kondo K., et al. Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity. Oncotarget 9, 34132 (2018); https://doi.org/10.18632/oncotarget.26139.
Abstract	Adoptive T-cell therapy with T cell receptor (TCR) -engineered T cells is an attractive strategy for cancer treatment and the success in this therapy is dependent on the functional avidity of the transduced TCRs against targeted tumor antigens. Therefore, the establishment of the methodology of the efficient and precise evaluation of TCR functional avidity has been awaited. Here, we show a novel platform cell line, named 2D3, which enables the functional avidity of transduced TCRs to be evaluated efficiently and precisely. In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling. Four different TCRs of HLA-A*24:02-restricted Wilms' tumor gene 1 (WT1)-specific CD8+ cytotoxic T lymphocytes (CTLs) were transduced into 2D3 cells and the functional avidities of these four TCRs were evaluated. The evaluated functional avidity of these TCRs positively correlated with cell proliferation, cytokine production, and WT1-specific cytotoxicity of the TCR-transduced CD8+ T cells in response to WT1 antigen. These results showed that 2D3 cell line was a novel and stable tool useful for the efficient and precise evaluation of the functional avidity of isolated and transduced TCRs in developing TCR-based immunotherapy.
Publisher	Impact Journals
Source Title	Oncotarget
Volume (Issue)	9(75)
Page	34132-34141
Date of Issued	2018-09-25
Language	English
Handle URL	https://hdl.handle.net/11094/98828
eISSN	19492553
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.18632/oncotarget.26139
Access Rights	open access
CopyRight	© Morimoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Oncotarget. 2018, 9(75), p. 34132-34141
DC.title	Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity
DC.creator	Morimoto, Soyoko
	Fujiki, Fumihiro
	Kondo, Kenta
	Nakajima, Hiroko
	Kobayashi, Yoshiki
	Inatome, Miki
	Aoyama, Nao
	Nishida, Yuya
	Tsuboi, Akihiro
	Oka, Yoshihiro
	Nishida, Sumiyuki
	Nakata, Jun
	Hosen, Naoki
	Oji, Yusuke
	Sugiyama, Haruo
DC.publisher	Impact Journals
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-09-25
DC.identifier	https://doi.org/10.18632/oncotarget.26139
DC.subject	TCR
	TCR functional avidity
	TCR-engineered T-cell therapy
	WT1
DC.description	Morimoto S., Fujiki F., Kondo K., et al. Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity. Oncotarget 9, 34132 (2018); https://doi.org/10.18632/oncotarget.26139.
DCTERMS.abstract	Adoptive T-cell therapy with T cell receptor (TCR) -engineered T cells is an attractive strategy for cancer treatment and the success in this therapy is dependent on the functional avidity of the transduced TCRs against targeted tumor antigens. Therefore, the establishment of the methodology of the efficient and precise evaluation of TCR functional avidity has been awaited. Here, we show a novel platform cell line, named 2D3, which enables the functional avidity of transduced TCRs to be evaluated efficiently and precisely. In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling. Four different TCRs of HLA-A*24:02-restricted Wilms' tumor gene 1 (WT1)-specific CD8+ cytotoxic T lymphocytes (CTLs) were transduced into 2D3 cells and the functional avidities of these four TCRs were evaluated. The evaluated functional avidity of these TCRs positively correlated with cell proliferation, cytokine production, and WT1-specific cytotoxicity of the TCR-transduced CD8+ T cells in response to WT1 antigen. These results showed that 2D3 cell line was a novel and stable tool useful for the efficient and precise evaluation of the functional avidity of isolated and transduced TCRs in developing TCR-based immunotherapy.
DC.rights	© Morimoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DC.format	98828
citation_title	Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity
citation_author	Morimoto, Soyoko
	Fujiki, Fumihiro
	Kondo, Kenta
	Nakajima, Hiroko
	Kobayashi, Yoshiki
	Inatome, Miki
	Aoyama, Nao
	Nishida, Yuya
	Tsuboi, Akihiro
	Oka, Yoshihiro
	Nishida, Sumiyuki
	Nakata, Jun
	Hosen, Naoki
	Oji, Yusuke
	Sugiyama, Haruo
citation_publisher	Impact Journals
citation_language	English
citation_date	2018-09-25
citation_journal_title	Oncotarget
citation_volume	9
citation_issue	75
citation_firstpage	34132
citation_lastpage	34141
citation_doi	https://doi.org/10.18632/oncotarget.26139
citation_public_url	https://hdl.handle.net/11094/98828

Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity

Link to primary information

Item Information

Show details