Generation of Replication-Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the Infection

Morita, Chiharu; Wada, Masami; Ohsaki, Eriko et al.

Microbiology and Immunology, 2024, 69(1), 43-58

Number of Access:657（2025-07-20 08:58 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98888

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Title
Generation of Replication-Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the Infection
Creator
CreatorName
Morita, Chiharu
NRID
1000000722654 1000000722654
researchmap permalink
masami-wada masami-wada
Scopus Author ID
36674132900 36674132900
CreatorName
Wada, Masami
NRID
1000050447801 1000050447801
ORCID
0000-0002-1496-0964 0000-0002-1496-0964
researchmap permalink
read0115382 read0115382
Scopus Author ID
6602478542 6602478542
CreatorName
Ohsaki, Eriko
NRID
1000070824184 1000070824184
researchmap permalink
ResearchSK ResearchSK
Scopus Author ID
36127298800 36127298800
CreatorName
Kimura-Ohba, Shihoko
NRID
1000000221797 1000000221797
researchmap permalink
read0076652 read0076652
Scopus Author ID
35367608100 35367608100
CreatorName
Ueda, Keiji
Subject
HBV polymerase
hepatitis B virus
recombinant virus
replication and infection competent
tagged HBV polymerase
Description
Morita C., Wada M., Ohsaki E., et al. Generation of Replication-Competent Hepatitis B Virus Harboring Tagged Polymerase for Visualization and Quantification of the Infection. Microbiology and Immunology, (2024); https://doi.org/10.1111/1348-0421.13183.
Abstract
Hepatitis B virus (HBV) infection is a serious global health problem causing acute and chronic hepatitis and related diseases. Approximately, 296 million patients have been chronically infected with the virus, leading to cirrhosis and hepatocellular carcinoma. Although HBV polymerase (HBVpol, pol) plays a pivotal role in HBV replication and must be a definite therapeutic target. The problems are that the detailed functions and intracellular dynamics of HBVpol remain unclear. Here, we constructed two kinds of tagged HBVpol, PA-tagged and HiBiT-tagged pol, and the HBV-producing vectors. Each PA tag and HiBiT tag were inserted into N-terminus of spacer region on HBVpol open reading frame. Transfection of the plasmids into HepG2 cells led to production of HBV. These tagged HBVpol were detectable in HBV replicating cells and pol-HiBiT enabled quantitative analysis. Furthermore, these recombinant HBV were infectious to primary human hepatocytes. Thus, we successfully designed infectious and replication-competent recombinant HBV harboring detectable tagged HBVpol. Such infectious recombinant HBV will provide a novel tool to study HBVpol dynamics and develop new therapeutics against HBV.
Publisher
John Wiley and Sons Inc
Source Title
Microbiology and Immunology
Volume (Issue)
69(1)
Page
43-58
Date of Issued
2024-12-02
Language
English
Handle URL
https://hdl.handle.net/11094/98888
PISSN
03855600
eISSN
13480421
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1111/1348-0421.13183
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article