New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center

Karino, Masaki; Yanagihara, Masashi; Harada, Tetsuya et al.

Microbiology Spectrum, 2025, 13(1), e02131-24

Number of Access:699（2025-08-30 09:57 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/99611

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Title
New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center
Creator
CreatorName
Karino, Masaki
NRID
1000040379954 1000040379954
researchmap permalink
7000022349 7000022349
CreatorName
Yanagihara, Masashi
NRID
1000070516723 1000070516723
CreatorName
Harada, Tetsuya
CreatorName
Sugo, Megumi
CreatorName
Karino, Mizuki
NRID
1000000733172 1000000733172
CreatorName
Ohtaki, Hirofumi
CreatorName
Hanada, Hiroyuki
NRID
1000000263236 1000000263236
researchmap permalink
read0185101 read0185101
CreatorName
Takano, Toru
CreatorName
Yamato, Masaya
NRID
1000050311759 1000050311759
ORCID
0000-0001-9359-380X 0000-0001-9359-380X
researchmap permalink
SOkamoto_20200212 SOkamoto_20200212
CreatorName
Okamoto, Shigefumi
Subject
Enterococcus faecium
vancomycin-resistant
multilocus sequence typing
pulsed-field gel electrophoresis
outbreak
Description
Copyright © American Society for Microbiology, Microbiology Spectrum 13(1), 2025, e02131-24; https://doi.org/10.1128/spectrum.02131-24.
Abstract
Vancomycin-resistant Enterococcus faecium (VREfm) is a major nosocomial pathogen, and molecular epidemiological tools are crucial for controlling its spread. Pulsed-field gel electrophoresis (PFGE) is still used in clinical laboratories despite the increased accessibility of whole-genome sequencing (WGS). As PFGE equipment is no longer commercially available, clinical laboratories need alternative tools. Highly standardized multilocus sequence typing (MLST) is one option. However, the original MLST scheme for E. faecium, designed in 2002, showed inconsistencies with WGS-based typing. Therefore, the new Bezdíček MLST scheme, which offers more accurate genetic similarity based on genome-wide data, has recently been proposed. To clarify its clinical utility in analyzing nosocomial VREfm transmission, we compared both MLST schemes with PFGE using 68 VREfm isolates collected during an outbreak at a Japanese tertiary medical center in 2019. PFGE analysis identified nine clusters among the 68 strains, including two predominant clusters. The original scheme identified five sequence types (STOs), of which 82.4% (56/68) were STO192. The Bezdíček scheme identified nine sequence types (STBs), subdividing the original STO192 into STB1162 (30/56), STB610 (25/56), and STB895 (1/56). Simpson’s index of diversity values were 0.635, 0.317, and 0.648 for PFGE, the original scheme, and the Bezdíček scheme, respectively. Combining the Bezdíček scheme with admission records provided clearer outbreak visualization, indicating that two distinct STBs independently caused sequential outbreaks. With high discriminatory power comparable with PFGE and global availability, the Bezdíček scheme is a practical and valuable tool for controlling nosocomial VREfm infections in clinical laboratories.
Publisher
American Society for Microbiology
Source Title
Microbiology Spectrum
Volume (Issue)
13(1)
Page
e02131-24
Date of Issued
2025-01-07
Language
English
Handle URL
https://hdl.handle.net/11094/99611
eISSN
21650497
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1128/spectrum.02131-24
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article