Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion

Furuichi, Takuya; Hirai, Hiromasa; Kitahara, Takayuki; Bun, Masayuki; Ikuta, Masato; Ukon, Yuichiro; Furuya, Masayuki; Oreffo, O.C. Richard; Janeczek, A. Agnieszka; Dawson, I. Jonathan; Okada, Seiji; Kaito, Takashi

doi:https://doi.org/10.1016/j.bioactmat.2024.10.027

Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion

Furuichi, Takuya; Hirai, Hiromasa; Kitahara, Takayuki et al.

Bioactive Materials, 2025, 44, 474-487

Number of Access:1,265（2025-07-24 10:22 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/99685

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Title	Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion
Creator	CreatorName Furuichi, Takuya
	CreatorName Hirai, Hiromasa
	CreatorName Kitahara, Takayuki
	CreatorName Bun, Masayuki
	CreatorName Ikuta, Masato
	NRID 1000050964334 1000050964334 ORCID 0000-0002-0747-8854 0000-0002-0747-8854 researchmap permalink yuichiro.ukon yuichiro.ukon CreatorName Ukon, Yuichiro
	NRID 1000020987466 1000020987466 researchmap permalink furuya1019 furuya1019 CreatorName Furuya, Masayuki
	CreatorName Oreffo, O.C. Richard
	CreatorName Janeczek, A. Agnieszka
	CreatorName Dawson, I. Jonathan
	NRID 1000030448435 1000030448435 ORCID 0000-0002-5107-8209 0000-0002-5107-8209 researchmap permalink okadaseiji okadaseiji Scopus Author ID 35079139100 35079139100 CreatorName Okada, Seiji
	NRID 1000070623982 1000070623982 ORCID 0000-0003-4882-2997 0000-0003-4882-2997 researchmap permalink 911_0 911_0 Scopus Author ID 25824322100 25824322100 CreatorName Kaito, Takashi
Subject	Bone morphogenetic protein 2
	Bone tissue engineering
	Endochondral ossification
	Inflammation
	Nanoclay
Description	Furuichi T., Hirai H., Kitahara T., et al. Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion. Bioactive Materials 44, 474 (2025); https://doi.org/10.1016/j.bioactmat.2024.10.027.
Abstract	Bone morphogenetic protein 2 (BMP2) is clinically applied for treating intractable fractures and promoting spinal fusion because of its osteogenic potency. However, adverse effects following the release of supraphysiological doses of BMP2 from collagen carriers are widely reported. Nanoclay gel (NC) is attracting attention as a biomaterial, given the potential for localized efficacy of administered agents. However, the efficacy and mechanism of action of NC/BMP2 remain unclear. This study explored the efficacy of NC as a BMP2 carrier in bone regeneration and the enhancement mechanism. Subfascial implantation of NC containing BMP2 elicited superior bone formation compared with collagen sponge (CS). Cartilage was uniformly formed inside the NC, whereas CS formed cartilage only on the perimeter. Additionally, CS induced a dose-dependent inflammatory response around the implantation site, whereas NC induced a minor response, and inflammatory cells were observed inside the NC. In a rat spinal fusion model, NC promoted high-quality bony fusion compared to CS. In vitro, NC enhanced chondrogenic and osteogenic differentiation of hBMSCs and ATDC5 cells while inhibiting osteoclastogenesis. Overall, NC/BMP2 facilitates spatially controlled, high-quality endochondral bone formation without BMP2-induced inflammation and promotes high-density new bone, functioning as a next-generation BMP2 carrier.
Publisher	KeAi Communications Co.
Source Title	Bioactive Materials
Volume (Issue)	44
Page	474-487
Date of Issued	2025-02-01
Language	English
Handle URL	https://hdl.handle.net/11094/99685
PISSN	2452199X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bioactmat.2024.10.027
Access Rights	open access
CopyRight	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Bioactive Materials. 2025, 44, p. 474-487
DC.title	Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion
DC.creator	Furuichi, Takuya
	Hirai, Hiromasa
	Kitahara, Takayuki
	Bun, Masayuki
	Ikuta, Masato
	Ukon, Yuichiro
	Furuya, Masayuki
	Oreffo, O.C. Richard
	Janeczek, A. Agnieszka
	Dawson, I. Jonathan
	Okada, Seiji
	Kaito, Takashi
DC.publisher	KeAi Communications Co.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-02-01
DC.identifier	https://doi.org/10.1016/j.bioactmat.2024.10.027
DC.subject	Bone morphogenetic protein 2
	Bone tissue engineering
	Endochondral ossification
	Inflammation
	Nanoclay
DC.description	Furuichi T., Hirai H., Kitahara T., et al. Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion. Bioactive Materials 44, 474 (2025); https://doi.org/10.1016/j.bioactmat.2024.10.027.
DCTERMS.abstract	Bone morphogenetic protein 2 (BMP2) is clinically applied for treating intractable fractures and promoting spinal fusion because of its osteogenic potency. However, adverse effects following the release of supraphysiological doses of BMP2 from collagen carriers are widely reported. Nanoclay gel (NC) is attracting attention as a biomaterial, given the potential for localized efficacy of administered agents. However, the efficacy and mechanism of action of NC/BMP2 remain unclear. This study explored the efficacy of NC as a BMP2 carrier in bone regeneration and the enhancement mechanism. Subfascial implantation of NC containing BMP2 elicited superior bone formation compared with collagen sponge (CS). Cartilage was uniformly formed inside the NC, whereas CS formed cartilage only on the perimeter. Additionally, CS induced a dose-dependent inflammatory response around the implantation site, whereas NC induced a minor response, and inflammatory cells were observed inside the NC. In a rat spinal fusion model, NC promoted high-quality bony fusion compared to CS. In vitro, NC enhanced chondrogenic and osteogenic differentiation of hBMSCs and ATDC5 cells while inhibiting osteoclastogenesis. Overall, NC/BMP2 facilitates spatially controlled, high-quality endochondral bone formation without BMP2-induced inflammation and promotes high-density new bone, functioning as a next-generation BMP2 carrier.
DC.rights	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
DC.format	99685
citation_title	Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion
citation_author	Furuichi, Takuya
	Hirai, Hiromasa
	Kitahara, Takayuki
	Bun, Masayuki
	Ikuta, Masato
	Ukon, Yuichiro
	Furuya, Masayuki
	Oreffo, O.C. Richard
	Janeczek, A. Agnieszka
	Dawson, I. Jonathan
	Okada, Seiji
	Kaito, Takashi
citation_publisher	KeAi Communications Co.
citation_language	English
citation_date	2025-02-01
citation_journal_title	Bioactive Materials
citation_volume	44
citation_firstpage	474
citation_lastpage	487
citation_doi	https://doi.org/10.1016/j.bioactmat.2024.10.027
citation_public_url	https://hdl.handle.net/11094/99685

Nanoclay gels attenuate BMP2-associated inflammation and promote chondrogenesis to enhance BMP2-spinal fusion

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