AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection

Murakawa, Tomokazu; Ito, Jumpei; Rusu, Mara Camelia; Taneike, Manabu; Omiya, Shigemiki; Moncayo-Arlandi, Javier; Nakanishi, Chiaki; Sugihara, Ryuta; Nishida, Hiroki; Mine, Kentaro; Fleck, Roland; Zhang, Min; Nishida, Kazuhiko; Shah, M. Ajay; Yamaguchi, Osamu; Sakata, Yasushi; Otsu, Kinya

doi:https://doi.org/10.1016/j.celrep.2024.115001

AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection

Murakawa, Tomokazu; Ito, Jumpei; Rusu, Mara Camelia et al.

Cell Reports, 2024, 43(12), 115001

Number of Access:40（2025-04-30 13:26 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/99686

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Title	AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection
Creator	NRID 1000050902194 1000050902194 researchmap permalink tmurakawa tmurakawa Scopus Author ID 55211690100 55211690100 CreatorName Murakawa, Tomokazu
	NRID 1000030897583 1000030897583 ORCID 0000-0001-5149-9962 0000-0001-5149-9962 researchmap permalink jumpei-ito jumpei-ito CreatorName Ito, Jumpei
	CreatorName Rusu, Mara Camelia
	NRID 1000030609756 1000030609756 ORCID 0000-0002-4550-091X 0000-0002-4550-091X researchmap permalink osaka_mt osaka_mt CreatorName Taneike, Manabu
	NRID 1000040920260 1000040920260 CreatorName Omiya, Shigemiki
	CreatorName Moncayo-Arlandi, Javier
	CreatorName Nakanishi, Chiaki
	CreatorName Sugihara, Ryuta
	CreatorName Nishida, Hiroki
	CreatorName Mine, Kentaro
	CreatorName Fleck, Roland
	CreatorName Zhang, Min
	CreatorName Nishida, Kazuhiko
	CreatorName Shah, M. Ajay
	NRID 1000090467580 1000090467580 researchmap permalink osamu-yamaguchi osamu-yamaguchi CreatorName Yamaguchi, Osamu
	NRID 1000000397671 1000000397671 ORCID 0000-0002-5618-4721 0000-0002-5618-4721 researchmap permalink C4589 C4589 Scopus Author ID 35395964700 35395964700 CreatorName Sakata, Yasushi
	NRID 1000020294051 1000020294051 researchmap permalink kinyaotsu kinyaotsu CreatorName Otsu, Kinya
Subject	Bcl2-L-13
	CP: Cell biology
	heart failure
	mitochondria
	mitophagy
Description	Murakawa T., Ito J., Rusu M.C., et al. AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection. Cell Reports 43, 115001 (2024); https://doi.org/10.1016/j.celrep.2024.115001.
Abstract	The accumulation of damaged mitochondria in the heart is associated with heart failure. Mitophagy is an autophagic degradation system that specifically targets damaged mitochondria. We have reported previously that Bcl2-like protein 13 (Bcl2-L-13) mediates mitophagy and mitochondrial fission in mammalian cells. However, the in vivo function of Bcl2-L-13 remains unclear. Here, we demonstrate that Bcl2-L-13-deficient mice and knockin mice, in which the phosphorylation site (Ser272) on Bcl2-L-13 was changed to Ala, showed left ventricular dysfunction in response to pressure overload. Attenuation of mitochondrial fission and mitophagy led to impairment of ATP production in these mouse hearts. In addition, we identified AMPKα2 as the kinase responsible for the phosphorylation of Bcl2-L-13 at Ser272. These results indicate that Bcl2-L-13 and its phosphorylation play an important role in maintaining cardiac function. Furthermore, the amplitude of stress-stimulated mitophagic activity could be modulated by AMPKα2.
Publisher	Elsevier B.V.
Source Title	Cell Reports
Volume (Issue)	43(12)
Page	115001
Date of Issued	2024-12-24
Language	English
Handle URL	https://hdl.handle.net/11094/99686
PISSN	26391856
eISSN	22111247
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.celrep.2024.115001
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Cell Reports. 2024, 43(12), p. 115001
DC.title	AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection
DC.creator	Murakawa, Tomokazu
	Ito, Jumpei
	Rusu, Mara Camelia
	Taneike, Manabu
	Omiya, Shigemiki
	Moncayo-Arlandi, Javier
	Nakanishi, Chiaki
	Sugihara, Ryuta
	Nishida, Hiroki
	Mine, Kentaro
	Fleck, Roland
	Zhang, Min
	Nishida, Kazuhiko
	Shah, M. Ajay
	Yamaguchi, Osamu
	Sakata, Yasushi
	Otsu, Kinya
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-12-24
DC.identifier	https://doi.org/10.1016/j.celrep.2024.115001
DC.subject	Bcl2-L-13
	CP: Cell biology
	heart failure
	mitochondria
	mitophagy
DC.description	Murakawa T., Ito J., Rusu M.C., et al. AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection. Cell Reports 43, 115001 (2024); https://doi.org/10.1016/j.celrep.2024.115001.
DCTERMS.abstract	The accumulation of damaged mitochondria in the heart is associated with heart failure. Mitophagy is an autophagic degradation system that specifically targets damaged mitochondria. We have reported previously that Bcl2-like protein 13 (Bcl2-L-13) mediates mitophagy and mitochondrial fission in mammalian cells. However, the in vivo function of Bcl2-L-13 remains unclear. Here, we demonstrate that Bcl2-L-13-deficient mice and knockin mice, in which the phosphorylation site (Ser272) on Bcl2-L-13 was changed to Ala, showed left ventricular dysfunction in response to pressure overload. Attenuation of mitochondrial fission and mitophagy led to impairment of ATP production in these mouse hearts. In addition, we identified AMPKα2 as the kinase responsible for the phosphorylation of Bcl2-L-13 at Ser272. These results indicate that Bcl2-L-13 and its phosphorylation play an important role in maintaining cardiac function. Furthermore, the amplitude of stress-stimulated mitophagic activity could be modulated by AMPKα2.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	99686
citation_title	AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection
citation_author	Murakawa, Tomokazu
	Ito, Jumpei
	Rusu, Mara Camelia
	Taneike, Manabu
	Omiya, Shigemiki
	Moncayo-Arlandi, Javier
	Nakanishi, Chiaki
	Sugihara, Ryuta
	Nishida, Hiroki
	Mine, Kentaro
	Fleck, Roland
	Zhang, Min
	Nishida, Kazuhiko
	Shah, M. Ajay
	Yamaguchi, Osamu
	Sakata, Yasushi
	Otsu, Kinya
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2024-12-24
citation_journal_title	Cell Reports
citation_volume	43
citation_issue	12
citation_firstpage	115001
citation_doi	https://doi.org/10.1016/j.celrep.2024.115001
citation_public_url	https://hdl.handle.net/11094/99686

AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection

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