Exploring Nile Red staining as an analytical tool for surface-oxidized microplastics

Idehara, Wakaba; Haga, Yuya; Tsujino, Hirofumi et al.

Environmental Research, 2025, 269, 120934

Number of Access:1,015（2025-07-23 00:55 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100576

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
EnvironRes_269_120934 pdf None 2.94 MB 58 2025.03.14  
Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Exploring Nile Red staining as an analytical tool for surface-oxidized microplastics
Creator
CreatorName
Idehara, Wakaba
NRID
1000030888863 1000030888863
ORCID
0000-0003-4148-2256 0000-0003-4148-2256
researchmap permalink
30888863 30888863
Scopus Author ID
57211023415 57211023415
CreatorName
Haga, Yuya
NRID
1000010707144 1000010707144
researchmap permalink
htsujino htsujino
Scopus Author ID
36667726200 36667726200
CreatorName
Tsujino, Hirofumi
CreatorName
Ikuno, Yudai
CreatorName
Manabe, Sota
CreatorName
Hokaku, Mii
NRID
1000020632318 1000020632318
ORCID
0000-0002-1808-7373 0000-0002-1808-7373
researchmap permalink
pikurin3 pikurin3
Scopus Author ID
8856988200 8856988200
CreatorName
Asahara, Haruyasu
NRID
1000020646757 1000020646757
researchmap permalink
read646757 read646757
Scopus Author ID
35603386200 35603386200
CreatorName
Higashisaka, Kazuma
NRID
1000050263306 1000050263306
researchmap permalink
ytsutsumi ytsutsumi
Scopus Author ID
7202474093 7202474093
CreatorName
Tsutsumi, Yasuo
Subject
Carbonyl index
Environmental monitoring
Fluorescence imaging
Microplastic analysis
Surface oxidation
Description
Idehara W., Haga Y., Tsujino H., et al. Exploring Nile Red staining as an analytical tool for surface-oxidized microplastics. Environmental Research 269, 120934 (2025); https://doi.org/10.1016/j.envres.2025.120934.
Abstract
Microplastics (MPs), defined as plastic particles smaller than 5 mm, have garnered considerable attention owing to their potential biological impact on human health. These particles exhibit a range of physicochemical properties, including size, shape, and surface oxidation. Nile Red is a prominent tool for detecting microplastics, enabling staining for dynamic analyses within biological systems. However, the efficacy of Nile Red staining for surface-oxidized MPs remains unclear. Therefore, we applied Nile Red dye to stain surface-oxidized polyethylene and polyvinyl chloride and observed that both materials were effectively stained, although the fluorescence intensity varied according to different hydrophobic dynamics. Imaging analysis revealed a correlation between the fluorescence intensity score and the degree of surface oxidation, as determined using the carbonyl index calculated from attenuated total reflection-Fourier transform infrared spectroscopy data. Collectively, these findings offer novel analytical approaches for investigating environmental MPs, enhancing our understanding of their behavior and impact.
Publisher
Elsevier Inc.
Source Title
Environmental Research
Volume (Issue)
269
Page
120934
Date of Issued
2025-03-15
Language
English
Handle URL
https://hdl.handle.net/11094/100576
PISSN
00139351
eISSN
10960953
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.envres.2025.120934
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article