Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes

Hikida, Minami; Kanamoto, Takashi; Tachi, Yoshihito; Ebina, Kosuke; Nakajima, Masahiro; Nakata, Ken

doi:https://doi.org/10.1016/j.job.2025.100653

Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes

Hikida, Minami; Kanamoto, Takashi; Tachi, Yoshihito et al.

Journal of Oral Biosciences, 2025, 67(2), 100653

Number of Access:309（2025-09-03 11:41 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101389

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Title	Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes
Creator	CreatorName Hikida, Minami
	NRID 1000020512049 1000020512049 researchmap permalink kanamoto kanamoto Scopus Author ID 7005012135 7005012135 CreatorName Kanamoto, Takashi
	CreatorName Tachi, Yoshihito
	NRID 1000070612076 1000070612076 ORCID 0000-0002-2426-1024 0000-0002-2426-1024 researchmap permalink k-ebina k-ebina Scopus Author ID 8415453300 8415453300 CreatorName Ebina, Kosuke
	NRID 1000010188964 1000010188964 CreatorName Nakajima, Masahiro
	NRID 1000000283747 1000000283747 researchmap permalink ken-nakata ken-nakata Scopus Author ID 7402151308 7402151308 CreatorName Nakata, Ken
Subject	Osteoarthritis
	Human articular chondrocytes
	Cyclic compression loading
	Interleukin-1β induction
Description	Hikida M., Kanamoto T., Tachi Y., et al. Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes. Journal of Oral Biosciences 67, 100653 (2025); https://doi.org/10.1016/j.job.2025.100653.
Abstract	Objectives: Mechanical and inflammatory stimuli are key factors in the pathophysiology of osteoarthritis (OA). However, the effects of mechanical stimulation on joint tissues and cells at the molecular level and the mechanisms of interaction after stimulation with inflammatory cytokines remains uninvestigated. Methods: Three-dimensional cyclic compression loading (CCL) was applied to human articular chondrocytes, and the expression of OA-related genes was analyzed using reverse transcription quantitative real-time polymerase chain reaction. Additionally, the effects of CCL after the chondrocytes were stimulated with interleukin (IL)-1β were evaluated. A DNA microarray assay was used to compare changes in gene expression after chondrocytes were stimulated with IL-1β and CCL was applied, and to search for pathways that are affected by CCL. Results: CCL of 40 kPa significantly upregulated the expression of IL-8, cyclooxygenase (COX)-2, nerve growth factor, matrix metalloproteinase (MMP)-1, and MMP-3. Transcription of IL-8, COX-2, and MMP-3 was synergistically promoted by CCL and IL-1β. The top 10 pathways enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes were not common in either group, except for the “cytokine-cytokine receptor interaction”. The “tumor necrosis factor signaling pathway” and the “nuclear factor-kappa B signaling pathway” in the IL-1β group and “cell cycle” and the “Hippo signaling pathway” in the CCL group were included. Conclusions: Comprehensive gene expression analysis revealed that CCL-induced changes in gene expression were different to those induced by stimulation with IL-1β. Our results provide new insights into the involvement of mechanical stimulation in the pathogenesis of OA.
Publisher	Japanese Association for Oral Biology
Source Title	Journal of Oral Biosciences
Volume (Issue)	67(2)
Page	100653
Date of Issued	2025-03-19
Language	English
Handle URL	https://hdl.handle.net/11094/101389
PISSN	13490079
eISSN	18803865
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.job.2025.100653
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Oral Biosciences. 2025, 67(2), p. 100653
DC.title	Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes
DC.creator	Hikida, Minami
	Kanamoto, Takashi
	Tachi, Yoshihito
	Ebina, Kosuke
	Nakajima, Masahiro
	Nakata, Ken
DC.publisher	Japanese Association for Oral Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-03-19
DC.identifier	https://doi.org/10.1016/j.job.2025.100653
DC.subject	Osteoarthritis
	Human articular chondrocytes
	Cyclic compression loading
	Interleukin-1β induction
DC.description	Hikida M., Kanamoto T., Tachi Y., et al. Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes. Journal of Oral Biosciences 67, 100653 (2025); https://doi.org/10.1016/j.job.2025.100653.
DCTERMS.abstract	Objectives: Mechanical and inflammatory stimuli are key factors in the pathophysiology of osteoarthritis (OA). However, the effects of mechanical stimulation on joint tissues and cells at the molecular level and the mechanisms of interaction after stimulation with inflammatory cytokines remains uninvestigated. Methods: Three-dimensional cyclic compression loading (CCL) was applied to human articular chondrocytes, and the expression of OA-related genes was analyzed using reverse transcription quantitative real-time polymerase chain reaction. Additionally, the effects of CCL after the chondrocytes were stimulated with interleukin (IL)-1β were evaluated. A DNA microarray assay was used to compare changes in gene expression after chondrocytes were stimulated with IL-1β and CCL was applied, and to search for pathways that are affected by CCL. Results: CCL of 40 kPa significantly upregulated the expression of IL-8, cyclooxygenase (COX)-2, nerve growth factor, matrix metalloproteinase (MMP)-1, and MMP-3. Transcription of IL-8, COX-2, and MMP-3 was synergistically promoted by CCL and IL-1β. The top 10 pathways enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes were not common in either group, except for the “cytokine-cytokine receptor interaction”. The “tumor necrosis factor signaling pathway” and the “nuclear factor-kappa B signaling pathway” in the IL-1β group and “cell cycle” and the “Hippo signaling pathway” in the CCL group were included. Conclusions: Comprehensive gene expression analysis revealed that CCL-induced changes in gene expression were different to those induced by stimulation with IL-1β. Our results provide new insights into the involvement of mechanical stimulation in the pathogenesis of OA.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	101389
citation_title	Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes
citation_author	Hikida, Minami
	Kanamoto, Takashi
	Tachi, Yoshihito
	Ebina, Kosuke
	Nakajima, Masahiro
	Nakata, Ken
citation_publisher	Japanese Association for Oral Biology
citation_language	English
citation_date	2025-03-19
citation_journal_title	Journal of Oral Biosciences
citation_volume	67
citation_issue	2
citation_firstpage	100653
citation_doi	https://doi.org/10.1016/j.job.2025.100653
citation_public_url	https://hdl.handle.net/11094/101389

Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction: Cyclic Compression on Human Chondrocytes

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