CRISPR/Cas9-mediated Genome-editing Reveals 10 Testis-enriched Genes and One Non-testis-enriched Gene are Dispensable for Male Fecundity in Mice

Qiu, Yumiao; Shimada, Keisuke; Ikawa, Masahito

Andrology, 2025

Number of Access:65（2026-02-05 22:24 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/103465

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Title
CRISPR/Cas9-mediated Genome-editing Reveals 10 Testis-enriched Genes and One Non-testis-enriched Gene are Dispensable for Male Fecundity in Mice
Creator
CreatorName
Qiu, Yumiao
NRID
1000060779601 1000060779601
ORCID
0000-0003-3739-7163 0000-0003-3739-7163
researchmap permalink
nununununu nununununu
Scopus Author ID
56479808000 56479808000
CreatorName
Shimada, Keisuke
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
Subject
CRISPR/Cas9
knockout mice
male infertility
spermatozoa
testis
Description
Qiu Y., Shimada K., Ikawa M.. CRISPR/Cas9-mediated Genome-editing Reveals 10 Testis-enriched Genes and One Non-testis-enriched Gene are Dispensable for Male Fecundity in Mice. Andrology , (2025); https://doi.org/10.1111/andr.70144.
Abstract
Background: More than 1000 genes have been identified as predominantly expressed in the human testis. Advances in gene editing technologies have enabled the rapid and efficient generation of genetically engineered mice. This approach facilitates the screening of genes essential for spermatogenesis by analyzing knockout mouse models. Objectives: This study aimed to elucidate the essential genes in male reproductive function by generating knockout mouse models. Materials and Methods: We selected 11 target genes that may have potential roles in the male reproductive system based on a public database. Knockout mouse lines of these target genes were generated using the CRISPR/Cas9 system to elucidate their functions in male reproduction. Also, we conducted natural mating tests to elucidate fecundity and analyzed the phenotype of the knockout males. Results: Natural mating tests revealed that all 11 gene-deficient mouse lines maintained normal male fertility. The phenotypic analysis, including testis appearance and weight, histology of testis and epididymis, and sperm motility and morphology, showed no apparent abnormalities. Discussion and Conclusion: These results suggest that each gene is not essential for male reproductive function.
Publisher
John Wiley and Sons Inc
Source Title
Andrology
Date of Issued
2025-11-10
Language
English
Handle URL
https://hdl.handle.net/11094/103465
PISSN
20472919
eISSN
20472927
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1111/andr.70144
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article