Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat

Ozasa, Ryosuke; Ishimoto, Takuya; Miyabe, Sayaka; Hashimoto, Jun; Hirao, Makoto; Yoshikawa, Hideki; Nakano, Takayoshi

doi:https://doi.org/10.1007/s00223-018-0508-z

Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat

Ozasa, Ryosuke; Ishimoto, Takuya; Miyabe, Sayaka et al.

Calcified Tissue International, 2018, 104, 449-460

Number of Access:696（2025-07-18 09:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89823

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Title	Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat
Creator	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000050584132 1000050584132 researchmap permalink read0151100 read0151100 Scopus Author ID 16239274400 16239274400 CreatorName Miyabe, Sayaka
	NRID 1000040237938 1000040237938 CreatorName Hashimoto, Jun
	NRID 1000010527708 1000010527708 CreatorName Hirao, Makoto
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Collagen/apatite orientation
	Osteocyte
	Osteoporosis
	Vertebral bone
	Young’s modulus
Description	Ozasa R., Ishimoto T., Miyabe S., et al. Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat. Calcified Tissue International, 104, 4, 449. https://doi.org/10.1007/s00223-018-0508-z.
Abstract	This study revealed the distinguished changes of preferential orientation of collagen and apatite and Young’s modulus in two different types of osteoporotic bones compared with the normal bone. Little is known about the bone material properties of osteoporotic bones; therefore, we aimed to assess material properties in osteoporotic bones. 66 female Sprague–Dawley rats were used. We analyzed the volumetric bone mineral density, collagen/apatite orientation, and Young’s modulus of fifth lumbar vertebral cortex for osteoporotic rats caused by ovariectomy (OVX), administration of low calcium and phosphate content (LCaP) diet, and their combination (OVX + LCaP), as well as sham-operated control. Osteocyte conditions were assessed by hematoxylin and eosin and immunohistochemical (matrix extracellular phosphoglycoprotein (MEPE) and dentin matrix protein 1 (DMP1)) staining. All osteoporotic animals showed bone loss compared with the sham-operated control. OVX improved craniocaudal Young’s modulus by enhancing collagen/apatite orientation along the craniocaudal axis, likely in response to the elevated stress due to osteoporotic bone loss. Conversely, LCaP-fed animals showed either significant bone loss or degraded collagen/apatite orientation and Young’s modulus. Osteocytes in LCaP and OVX + LCaP groups showed atypical appearance and MEPE- and DMP1-negative phenotype, whereas those in the OVX group showed similarity with osteocytes in the control group. This suggests that osteocytes are possibly involved in the osteoporotic changes in collagen/apatite orientation and Young’s modulus. This study is the first to demonstrate that osteoporosis changes collagen/apatite orientation and Young’s modulus in an opposite manner depending on the cause of osteoporosis in spite of common bone loss.
Publisher	Springer
Source Title	Calcified Tissue International
Volume (Issue)	104
Page	449-460
Date of Issued	2018-12-26
Language	English
Handle URL	https://hdl.handle.net/11094/89823
PISSN	0171967X
eISSN	14320827
NCID	AA00141085
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1007/s00223-018-0508-z
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Calcified Tissue International. 2018, 104, p. 449-460
DC.title	Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat
DC.creator	Ozasa, Ryosuke
	Ishimoto, Takuya
	Miyabe, Sayaka
	Hashimoto, Jun
	Hirao, Makoto
	Yoshikawa, Hideki
	Nakano, Takayoshi
DC.publisher	Springer
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-12-26
DC.identifier	https://doi.org/10.1007/s00223-018-0508-z
DC.subject	Collagen/apatite orientation
	Osteocyte
	Osteoporosis
	Vertebral bone
	Young’s modulus
DC.description	Ozasa R., Ishimoto T., Miyabe S., et al. Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat. Calcified Tissue International, 104, 4, 449. https://doi.org/10.1007/s00223-018-0508-z.
DCTERMS.abstract	This study revealed the distinguished changes of preferential orientation of collagen and apatite and Young’s modulus in two different types of osteoporotic bones compared with the normal bone. Little is known about the bone material properties of osteoporotic bones; therefore, we aimed to assess material properties in osteoporotic bones. 66 female Sprague–Dawley rats were used. We analyzed the volumetric bone mineral density, collagen/apatite orientation, and Young’s modulus of fifth lumbar vertebral cortex for osteoporotic rats caused by ovariectomy (OVX), administration of low calcium and phosphate content (LCaP) diet, and their combination (OVX + LCaP), as well as sham-operated control. Osteocyte conditions were assessed by hematoxylin and eosin and immunohistochemical (matrix extracellular phosphoglycoprotein (MEPE) and dentin matrix protein 1 (DMP1)) staining. All osteoporotic animals showed bone loss compared with the sham-operated control. OVX improved craniocaudal Young’s modulus by enhancing collagen/apatite orientation along the craniocaudal axis, likely in response to the elevated stress due to osteoporotic bone loss. Conversely, LCaP-fed animals showed either significant bone loss or degraded collagen/apatite orientation and Young’s modulus. Osteocytes in LCaP and OVX + LCaP groups showed atypical appearance and MEPE- and DMP1-negative phenotype, whereas those in the OVX group showed similarity with osteocytes in the control group. This suggests that osteocytes are possibly involved in the osteoporotic changes in collagen/apatite orientation and Young’s modulus. This study is the first to demonstrate that osteoporosis changes collagen/apatite orientation and Young’s modulus in an opposite manner depending on the cause of osteoporosis in spite of common bone loss.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89823
citation_title	Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat
citation_author	Ozasa, Ryosuke
	Ishimoto, Takuya
	Miyabe, Sayaka
	Hashimoto, Jun
	Hirao, Makoto
	Yoshikawa, Hideki
	Nakano, Takayoshi
citation_publisher	Springer
citation_language	English
citation_date	2018-12-26
citation_journal_title	Calcified Tissue International
citation_volume	104
citation_firstpage	449
citation_lastpage	460
citation_doi	https://doi.org/10.1007/s00223-018-0508-z
citation_public_url	https://hdl.handle.net/11094/89823

Osteoporosis Changes Collagen/Apatite Orientation and Young’s Modulus in Vertebral Cortical Bone of Rat

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