Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction

Nakajima, Kichitaro; Toda, Hajime; Yamaguchi, Keiichi; So, Masatomo; Ikenaka, Kensuke; Mochizuki, Hideki; Goto, Yuji; Ogi, Hirotsugu

doi:https://doi.org/10.1021/acschemneuro.1c00461

Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction

Nakajima, Kichitaro; Toda, Hajime; Yamaguchi, Keiichi et al.

ACS Chemical Neuroscience, 2021, 12(18), 3456-3466

Number of Access:1,197（2025-08-13 09:09 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93313

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Title	Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction
Creator	NRID 1000020867337 1000020867337 ORCID 0000-0003-2644-741X 0000-0003-2644-741X researchmap permalink sonochem sonochem Scopus Author ID 57145904700 57145904700 CreatorName Nakajima, Kichitaro
	CreatorName Toda, Hajime
	NRID 1000090432187 1000090432187 researchmap permalink read0105908 read0105908 Scopus Author ID 8731908100 8731908100 CreatorName Yamaguchi, Keiichi
	NRID 1000040746335 1000040746335 CreatorName So, Masatomo
	NRID 1000070774058 1000070774058 researchmap permalink kensukeikenaka kensukeikenaka Scopus Author ID 8588484300 8588484300 CreatorName Ikenaka, Kensuke
	NRID 1000090230044 1000090230044 ORCID 0000-0002-0874-7542 0000-0002-0874-7542 researchmap permalink read0207787 read0207787 Scopus Author ID 7202006099 7202006099 CreatorName Mochizuki, Hideki
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 CreatorName Ogi, Hirotsugu
Subject	amyloid fibril
	seed detection
	shaking
	supersaturation
	ultrasonic cavitation
	ultrasonication
Description	Nakajima K., Toda H., Yamaguchi K., et al. Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction. ACS Chemical Neuroscience 12, 3456 (2021); https://doi.org/10.1021/acschemneuro.1c00461.
Abstract	Ultrasonication has been recently adopted in amyloid-fibril assays because of its ability to accelerate fibril formation, being promising in the early stage diagnosis of amyloidoses in clinical applications. Although applications of this technique are expanding in the field of protein science, its effects on the aggregation reactions of amyloidogenic proteins are poorly understood. In this study, we comprehensively investigated the morphology and structure of resultant aggregates, kinetics of fibril formation, and seed-detection sensitivity under ultrasonication using β2-microglobulin and compared these characteristics under shaking, which has been traditionally adopted in amyloid-fibril assays. To discuss the ultrasonic effects on the amyloid-fibril formation, we propose the half-time heat map, which describes the phase diagram of the aggregation reaction of amyloidogenic proteins. The experimental results show that ultrasonication greatly promotes fibril formation, especially in dilute monomer solutions, induces short-dispersed fibrils, and is capable of detecting ultra-trace-concentration seeds with a detection limit of 10 fM. Furthermore, we indicate that ultrasonication highly alters the energy landscape of an aggregation reaction due to the effect of ultrasonic cavitation. These insights contribute not only to our understanding of the effects of agitation on amyloidogenic aggregation reactions but also to their effective application in the clinical diagnosis of amyloidoses.
Publisher	American Chemical Society
Source Title	ACS Chemical Neuroscience
Volume (Issue)	12(18)
Page	3456-3466
Date of Issued	2021-09-15
Language	English
Handle URL	https://hdl.handle.net/11094/93313
eISSN	19487193
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1021/acschemneuro.1c00461
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Neuroscience, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acschemneuro.1c00461.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	ACS Chemical Neuroscience. 2021, 12(18), p. 3456-3466
DC.title	Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction
DC.creator	Nakajima, Kichitaro
	Toda, Hajime
	Yamaguchi, Keiichi
	So, Masatomo
	Ikenaka, Kensuke
	Mochizuki, Hideki
	Goto, Yuji
	Ogi, Hirotsugu
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-09-15
DC.identifier	https://doi.org/10.1021/acschemneuro.1c00461
DC.subject	amyloid fibril
	seed detection
	shaking
	supersaturation
	ultrasonic cavitation
	ultrasonication
DC.description	Nakajima K., Toda H., Yamaguchi K., et al. Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction. ACS Chemical Neuroscience 12, 3456 (2021); https://doi.org/10.1021/acschemneuro.1c00461.
DCTERMS.abstract	Ultrasonication has been recently adopted in amyloid-fibril assays because of its ability to accelerate fibril formation, being promising in the early stage diagnosis of amyloidoses in clinical applications. Although applications of this technique are expanding in the field of protein science, its effects on the aggregation reactions of amyloidogenic proteins are poorly understood. In this study, we comprehensively investigated the morphology and structure of resultant aggregates, kinetics of fibril formation, and seed-detection sensitivity under ultrasonication using β2-microglobulin and compared these characteristics under shaking, which has been traditionally adopted in amyloid-fibril assays. To discuss the ultrasonic effects on the amyloid-fibril formation, we propose the half-time heat map, which describes the phase diagram of the aggregation reaction of amyloidogenic proteins. The experimental results show that ultrasonication greatly promotes fibril formation, especially in dilute monomer solutions, induces short-dispersed fibrils, and is capable of detecting ultra-trace-concentration seeds with a detection limit of 10 fM. Furthermore, we indicate that ultrasonication highly alters the energy landscape of an aggregation reaction due to the effect of ultrasonic cavitation. These insights contribute not only to our understanding of the effects of agitation on amyloidogenic aggregation reactions but also to their effective application in the clinical diagnosis of amyloidoses.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Neuroscience, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acschemneuro.1c00461.
DC.format	93313
citation_title	Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction
citation_author	Nakajima, Kichitaro
	Toda, Hajime
	Yamaguchi, Keiichi
	So, Masatomo
	Ikenaka, Kensuke
	Mochizuki, Hideki
	Goto, Yuji
	Ogi, Hirotsugu
citation_publisher	American Chemical Society
citation_language	English
citation_date	2021-09-15
citation_journal_title	ACS Chemical Neuroscience
citation_volume	12
citation_issue	18
citation_firstpage	3456
citation_lastpage	3466
citation_doi	https://doi.org/10.1021/acschemneuro.1c00461
citation_public_url	https://hdl.handle.net/11094/93313

Half-Time Heat Map Reveals Ultrasonic Effects on Morphology and Kinetics of Amyloidogenic Aggregation Reaction

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