An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond

Asai, Kai; Yamamoto, Takashi; Nagashima, Shinichi; Ogata, Genki; Hibino, Hiroshi; Einaga, Yasuaki

doi:https://doi.org/10.1039/c9an01976f

An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond

Asai, Kai; Yamamoto, Takashi; Nagashima, Shinichi et al.

Analyst, 2020, 145(2), 544-549

Number of Access:648（2025-10-10 20:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93344

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Title	An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond
Creator	CreatorName Asai, Kai
	NRID 1000040532908 1000040532908 CreatorName Yamamoto, Takashi
	CreatorName Nagashima, Shinichi
	NRID 1000080452829 1000080452829 CreatorName Ogata, Genki
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 CreatorName Hibino, Hiroshi
	NRID 1000000322066 1000000322066 CreatorName Einaga, Yasuaki
Description	Reproduced from Analyst., 2020, 145, 544-549 with permission from the Royal Society of Chemistry.
Description	https://doi.org/10.1039/c9an01976f
Abstract	Stable and continuous biosensing of electroactive species in vivo has been achieved by using boron-doped diamond (BDD) electrodes owing to their outstanding electrochemical properties. However, the present problem in biosensing using BDD electrodes is how to specifically measure/detect the target molecules, including electrochemically inactive species. A possible solution is to fabricate an electrochemical aptamer-based (E-AB) sensor using a BDD electrode. In a preliminary investigation, we found that DNA aptamers strongly adsorb on the BDD surface and the aptamer-adsorbed BDD apparently worked as an E-AB sensor. The present study reports the performance of the aptamer-adsorbed BDD electrode as an E-AB sensor. Doxorubicin (DOX), a widely used chemotherapeutic, was chosen as a target molecule. The sensor could be prepared by just dipping BDD in an aptamer solution for only 30 min, and the electrochemical signals were dependent on the DOX concentration. The adsorption of DNA was strong enough for continuous measurements and even a sonication treatment. Such behaviors were not observed when using gold and glassy carbon electrodes. In a kinetic measurement, distortion by a sluggish response was observed for both association and dissociation phases, indicating that the interaction between DOX and the aptamer involves several kinetic processes. By fitting to a Langmuir isotherm, a limit of detection of 49 nM and a maximum detectable concentration of 2.3 μM were obtained. Although the sensitivity was lower than those of the well-established E-AB sensors of gold, the values are within a drug's therapeutic range. Overall, the present work demonstrates that a DNA aptamer and a BDD electrode is an effective combination for an E-AB sensor with stable sensitivity, and a wide variety of DNA aptamers can be applied without any special treatment.
Publisher	Royal Society of Chemistry
Source Title	Analyst
Volume (Issue)	145(2)
Page	544-549
Date of Issued	2020-01-21
Language	English
Handle URL	https://hdl.handle.net/11094/93344
PISSN	00032654
eISSN	13645528
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1039/c9an01976f
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analyst. 2020, 145(2), p. 544-549
DC.title	An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond
DC.creator	Asai, Kai
	Yamamoto, Takashi
	Nagashima, Shinichi
	Ogata, Genki
	Hibino, Hiroshi
	Einaga, Yasuaki
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-01-21
DC.identifier	https://doi.org/10.1039/c9an01976f
DC.description	Reproduced from Analyst., 2020, 145, 544-549 with permission from the Royal Society of Chemistry.
DC.description	https://doi.org/10.1039/c9an01976f
DCTERMS.abstract	Stable and continuous biosensing of electroactive species in vivo has been achieved by using boron-doped diamond (BDD) electrodes owing to their outstanding electrochemical properties. However, the present problem in biosensing using BDD electrodes is how to specifically measure/detect the target molecules, including electrochemically inactive species. A possible solution is to fabricate an electrochemical aptamer-based (E-AB) sensor using a BDD electrode. In a preliminary investigation, we found that DNA aptamers strongly adsorb on the BDD surface and the aptamer-adsorbed BDD apparently worked as an E-AB sensor. The present study reports the performance of the aptamer-adsorbed BDD electrode as an E-AB sensor. Doxorubicin (DOX), a widely used chemotherapeutic, was chosen as a target molecule. The sensor could be prepared by just dipping BDD in an aptamer solution for only 30 min, and the electrochemical signals were dependent on the DOX concentration. The adsorption of DNA was strong enough for continuous measurements and even a sonication treatment. Such behaviors were not observed when using gold and glassy carbon electrodes. In a kinetic measurement, distortion by a sluggish response was observed for both association and dissociation phases, indicating that the interaction between DOX and the aptamer involves several kinetic processes. By fitting to a Langmuir isotherm, a limit of detection of 49 nM and a maximum detectable concentration of 2.3 μM were obtained. Although the sensitivity was lower than those of the well-established E-AB sensors of gold, the values are within a drug's therapeutic range. Overall, the present work demonstrates that a DNA aptamer and a BDD electrode is an effective combination for an E-AB sensor with stable sensitivity, and a wide variety of DNA aptamers can be applied without any special treatment.
DC.format	93344
citation_title	An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond
citation_author	Asai, Kai
	Yamamoto, Takashi
	Nagashima, Shinichi
	Ogata, Genki
	Hibino, Hiroshi
	Einaga, Yasuaki
citation_publisher	Royal Society of Chemistry
citation_language	English
citation_date	2020-01-21
citation_journal_title	Analyst
citation_volume	145
citation_issue	2
citation_firstpage	544
citation_lastpage	549
citation_doi	https://doi.org/10.1039/c9an01976f
citation_public_url	https://hdl.handle.net/11094/93344

An electrochemical aptamer-based sensor prepared by utilizing the strong interaction between a DNA aptamer and diamond

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