Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma

Jingushi, Kentaro; Kawashima, Atsunari; Tanikawa, Sayaka; Saito, Takuro; Yamamoto, Akinaru; Uemura, Toshihiro; Sassi, Nesrine; Ishizuya, Yu; Yamamoto, Yoshiyuki; Kato, Taigo; Hatano, Koji; Hase, Hiroaki; Nonomura, Norio; Tsujikawa, Kazutake

doi:https://doi.org/10.1111/cas.16202

Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma

Jingushi, Kentaro; Kawashima, Atsunari; Tanikawa, Sayaka et al.

Cancer Science, 2024, 115(8), 2578-2587

Number of Access:2,144（2025-08-16 04:45 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/97094

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Title	Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma
Creator	NRID 1000080707571 1000080707571 ORCID 0000-0002-2274-4962 0000-0002-2274-4962 researchmap permalink _kj _kj Scopus Author ID 13807151700 13807151700 CreatorName Jingushi, Kentaro
	NRID 1000050746568 1000050746568 researchmap permalink akawashima akawashima Scopus Author ID 9846139400 9846139400 CreatorName Kawashima, Atsunari
	CreatorName Tanikawa, Sayaka
	NRID 1000020646468 1000020646468 researchmap permalink tsaito19 tsaito19 Scopus Author ID 57211629912 57211629912 CreatorName Saito, Takuro
	NRID 1000050909653 1000050909653 researchmap permalink ayamamoto ayamamoto ORCID 0000-0002-3565-5870 0000-0002-3565-5870 CreatorName Yamamoto, Akinaru
	NRID 1000060905941 1000060905941 CreatorName Uemura, Toshihiro
	CreatorName Sassi, Nesrine
	NRID 1000000783854 1000000783854 researchmap permalink okiishi okiishi CreatorName Ishizuya, Yu
	NRID 1000090759557 1000090759557 researchmap permalink yoshi229 yoshi229 ORCID 0000-0002-1271-3521 0000-0002-1271-3521 CreatorName Yamamoto, Yoshiyuki
	NRID 1000070648021 1000070648021 researchmap permalink taigokato taigokato Scopus Author ID 55414697500 55414697500 ORCID 0000-0002-8681-1407 0000-0002-8681-1407 CreatorName Kato, Taigo
	NRID 1000060762234 1000060762234 researchmap permalink koji_hatano koji_hatano Scopus Author ID 14627167600 14627167600 CreatorName Hatano, Koji
	NRID 1000080779926 1000080779926 researchmap permalink hase-h hase-h Scopus Author ID 55360273600 55360273600 CreatorName Hase, Hiroaki
	NRID 1000030263263 1000030263263 researchmap permalink read0185096 read0185096 Scopus Author ID 7005880662 7005880662 CreatorName Nonomura, Norio
	NRID 1000010207376 1000010207376 researchmap permalink read0014359 read0014359 Scopus Author ID 7004354760 7004354760 CreatorName Tsujikawa, Kazutake
Subject	16S rRNA gene
	bacterial DNA
	Cutibacterium acnes
	extracellular vesicles
	renal cell carcinoma
Description	Jingushi K., Kawashima A., Tanikawa S., et al. Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma. Cancer Science, (2024); https://doi.org/10.1111/cas.16202.
Abstract	Bacterial flora are present in various parts of the human body, including the intestine, and are thought to be involved in the etiology of various diseases such as multiple sclerosis, intestinal diseases, cancer, and uterine diseases. In recent years, the presence of bacterial 16S rRNA genes has been revealed in blood, which was previously thought to be a sterile environment, and characteristic blood microbiomes have been detected in various diseases. However, the mechanism and the origin of the bacterial information are unknown. In this study, we performed 16S rRNA metagenomic analysis of bacterial DNA in serum extracellular vesicles from five healthy donors and seven patients with renal cell carcinoma and detected Cutibacterium acnes DNA as a characteristic bacterial DNA in the serum extracellular vesicles of patients with renal cell carcinoma. In addition, C. acnes DNA was significantly reduced in postoperative serum extracellular vesicles from patients with renal cell carcinoma compared with that in preoperative serum extracellular vesicles from these patients and was also detected in tumor tissue and extracellular vesicles from tumor tissue-associated microbiota, suggesting an association between C. acnes extracellular vesicles and renal cell carcinoma. C. acnes extracellular vesicles were taken up by renal carcinoma cells to enhance their proliferative potential. C. acnes extracellular vesicles also exhibited tumor-promoting activity in a mouse model of renal cancer allografts with enhanced angiogenesis. These results suggest that extracellular vesicles released by C. acnes localized in renal cell carcinoma tissues act in a tumor-promoting manner.
Publisher	John Wiley and Sons Inc
Source Title	Cancer Science
Volume (Issue)	115(8)
Page	2578-2587
Date of Issued	2024-04-29
Language	English
Handle URL	https://hdl.handle.net/11094/97094
PISSN	13479032
eISSN	13497006
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1111/cas.16202
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Cancer Science. 2024, 115(8), p. 2578-2587
DC.title	Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma
DC.creator	Jingushi, Kentaro
	Kawashima, Atsunari
	Tanikawa, Sayaka
	Saito, Takuro
	Yamamoto, Akinaru
	Uemura, Toshihiro
	Sassi, Nesrine
	Ishizuya, Yu
	Yamamoto, Yoshiyuki
	Kato, Taigo
	Hatano, Koji
	Hase, Hiroaki
	Nonomura, Norio
	Tsujikawa, Kazutake
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-04-29
DC.identifier	https://doi.org/10.1111/cas.16202
DC.subject	16S rRNA gene
	bacterial DNA
	Cutibacterium acnes
	extracellular vesicles
	renal cell carcinoma
DC.description	Jingushi K., Kawashima A., Tanikawa S., et al. Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma. Cancer Science, (2024); https://doi.org/10.1111/cas.16202.
DCTERMS.abstract	Bacterial flora are present in various parts of the human body, including the intestine, and are thought to be involved in the etiology of various diseases such as multiple sclerosis, intestinal diseases, cancer, and uterine diseases. In recent years, the presence of bacterial 16S rRNA genes has been revealed in blood, which was previously thought to be a sterile environment, and characteristic blood microbiomes have been detected in various diseases. However, the mechanism and the origin of the bacterial information are unknown. In this study, we performed 16S rRNA metagenomic analysis of bacterial DNA in serum extracellular vesicles from five healthy donors and seven patients with renal cell carcinoma and detected Cutibacterium acnes DNA as a characteristic bacterial DNA in the serum extracellular vesicles of patients with renal cell carcinoma. In addition, C. acnes DNA was significantly reduced in postoperative serum extracellular vesicles from patients with renal cell carcinoma compared with that in preoperative serum extracellular vesicles from these patients and was also detected in tumor tissue and extracellular vesicles from tumor tissue-associated microbiota, suggesting an association between C. acnes extracellular vesicles and renal cell carcinoma. C. acnes extracellular vesicles were taken up by renal carcinoma cells to enhance their proliferative potential. C. acnes extracellular vesicles also exhibited tumor-promoting activity in a mouse model of renal cancer allografts with enhanced angiogenesis. These results suggest that extracellular vesicles released by C. acnes localized in renal cell carcinoma tissues act in a tumor-promoting manner.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	97094
citation_title	Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma
citation_author	Jingushi, Kentaro
	Kawashima, Atsunari
	Tanikawa, Sayaka
	Saito, Takuro
	Yamamoto, Akinaru
	Uemura, Toshihiro
	Sassi, Nesrine
	Ishizuya, Yu
	Yamamoto, Yoshiyuki
	Kato, Taigo
	Hatano, Koji
	Hase, Hiroaki
	Nonomura, Norio
	Tsujikawa, Kazutake
citation_publisher	John Wiley and Sons Inc
citation_language	English
citation_date	2024-04-29
citation_journal_title	Cancer Science
citation_volume	115
citation_issue	8
citation_firstpage	2578
citation_lastpage	2587
citation_doi	https://doi.org/10.1111/cas.16202
citation_public_url	https://hdl.handle.net/11094/97094

Cutibacterium acnes-derived extracellular vesicles promote tumor growth in renal cell carcinoma

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