Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

Zhang, Jingbo; Li, Junjun; Qu, Xiang et al.

Bioactive Materials, 2024, 37, 533-548

Number of Access:1,128（2025-08-15 18:59 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/98538

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Title
Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
Creator
CreatorName
Zhang, Jingbo
NRID
1000010723786 1000010723786
ORCID
0000-0002-7473-4918 0000-0002-7473-4918
researchmap permalink
liJJ liJJ
Scopus Author ID
52464043600 52464043600
CreatorName
Li, Junjun
CreatorName
Qu, Xiang
CreatorName
Liu, Yuting
CreatorName
Sun, Lifu
NRID
1000070535426 1000070535426
CreatorName
Harada, Akima
CreatorName
Hua, Ying
NRID
1000030432579 1000030432579
researchmap permalink
SougawaNagako SougawaNagako
CreatorName
Sougawa, Nagako
CreatorName
Tabata, Akiko
NRID
1000050380093 1000050380093
researchmap permalink
7000008694 7000008694
Scopus Author ID
55628530013 55628530013
CreatorName
Liu, Li
NRID
1000070544237 1000070544237
ORCID
0000-0003-0015-6569 0000-0003-0015-6569
researchmap permalink
s-miyagawa3154 s-miyagawa3154
Scopus Author ID
55560624700 55560624700
CreatorName
Miyagawa, Shigeru
Subject
Human adipose-derived mesenchymal stem cell
Human induced pluripotent stem cell-derived cardiomyocyte
Myocardial infarction
Regenerative medicine
Tissue engineering
Description
Zhang J., Li J., Qu X., et al. Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction. Bioactive Materials 37, 533 (2024); https://doi.org/10.1016/j.bioactmat.2024.03.028.
Abstract
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been widely used in therapy of ischemic heart disease. However, there are still remaining issues that limit the therapeutic efficacy, such as immune rejection and low retention of hiPSC-CMs. Human adipose mesenchymal stromal cells (hADSCs) have been reported to be able to regulate the immune response, promote angiogenesis and promote the maturation of hiPSC-CMs. In this study, we co-cultured these two types of cells on fiber scaffold made of biodegradable poly (D,L-lactic-co-glycolic acid) (PLGA) polymer for several days to develop a composited 3D cardiac tissue sheet. As expected, the cells formed 231.00 ± 15.14 μm thickness tissue, with improved organization, alignment, ECM condition, contractile ability, and paracrine function compared to culture hiPSC-CMs only on PLGA fiber. Furthermore, the composited 3D cardiac tissue sheet significantly promoted the engraftment and survival after transplantation. The composited 3D cardiac tissue sheet also increased cardiac function, attenuated ventricular remodeling, decreased fibrosis, and enhanced angiogenesis in rat myocardial infarction model, indicating that this strategy wound be a promising therapeutic option in the clinical scenario.
Publisher
Elsevier B.V.
Source Title
Bioactive Materials
Volume (Issue)
37
Page
533-548
Date of Issued
2024-07-01
Language
English
Handle URL
https://hdl.handle.net/11094/98538
PISSN
2452199X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.bioactmat.2024.03.028
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article